Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 2,000,294 A→G noncoding (404/2926 nt) SAUSA300_RS10040 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,000,2940AG97.7% 58.2 / ‑4.8 43noncoding (404/2926 nt)SAUSA300_RS1004023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (41/1);  total (41/2)
Fisher's exact test for biased strand distribution p-value = 4.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

TTCACTATCGGTCACTAGAGAGTATTTAGCCTTAGGAGATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAAGAGAGACAACATTTTCGACTACAGGATTATTACCTTCTTTGATTCATCTTTCCAGATGATTCGTCTAATGTC  >  NC_007793/2000197‑2000366
                                                                                                 |                                                                        
tttgcTATCGGTCACTAGAGAGTATTTAGCCTTAGGAGATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAagg                                                                        <  1:3194600/96‑2 (MQ=17)
 tCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTgaggag                                                                       >  1:1178917/1‑100 (MQ=11)
 tCACTATCGGTCACTAGAGAGTATTTAGCCTTAGGAGATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAagag                                                                       <  1:2582828/100‑1 (MQ=32)
  cACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAggaga                                                                      >  1:2892153/1‑100 (MQ=11)
      aggggTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaac                                                                  >  1:5349494/4‑98 (MQ=11)
        cGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAggagatcggaa                                                                >  1:3891825/1‑94 (MQ=11)
        cGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagatcgg                                                                >  1:1203417/1‑96 (MQ=11)
        cGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagatcgg                                                                >  1:6019464/1‑96 (MQ=11)
        cGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgacagatcgg                                                                >  1:3708010/1‑96 (MQ=11)
        cGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGACCCACTCGGgagagatcgg                                                                >  1:3717218/1‑96 (MQ=11)
        cGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGCCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTGAGgagagatcgg                                                                >  1:1275240/1‑96 (MQ=11)
        cGGTCACTAGGGAGTATTTAGCCTTGCGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCTGgagagatcgg                                                                >  1:190647/1‑96 (MQ=11)
        cGGTCACTAGAGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCCGgagagatcgg                                                                >  1:3092343/1‑96 (MQ=11)
        atctCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacga                                                                >  1:373623/4‑96 (MQ=11)
         tgTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagatcgga                                                               >  1:3728990/2‑95 (MQ=11)
          gccACTAGGGAATATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacgaag                                                              >  1:4182283/3‑94 (MQ=11)
          gTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCTGgagagatcggaa                                                              >  1:3778917/1‑94 (MQ=11)
          gTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagatcggaa                                                              >  1:1241669/1‑94 (MQ=11)
          gTCACTAGGGAGTATTTAGCCTTAGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCTGgagagatcggac                                                              >  1:4303112/1‑94 (MQ=11)
           ccACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacgaagt                                                             >  1:4460257/2‑93 (MQ=11)
            cACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgcgagatcggaaga                                                            >  1:6339887/1‑92 (MQ=11)
            cACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacgaagtt                                                            >  1:558801/1‑92 (MQ=11)
            cACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacgaagtt                                                            >  1:3315205/1‑92 (MQ=11)
            cACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacgaagtt                                                            >  1:6352830/1‑92 (MQ=11)
            cACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacgaagtt                                                            >  1:1573898/1‑92 (MQ=11)
            cACTAGGGAGTATTTAGCCTTGGGAGATGGTCCT‑‑CGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacgaagtttt                                                          >  1:1906961/1‑90 (MQ=11)
              cTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGgagagaacgaagtttt                                                          >  1:2151714/1‑90 (MQ=11)
                       aTTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgg                                                 >  1:2042896/1‑100 (MQ=11)
                       aTTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAga                                                 >  1:1718859/1‑99 (MQ=11)
                       aTTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACATGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgg                                                 >  1:2371059/1‑100 (MQ=11)
                        ttcaGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAggg                                                >  1:2840595/4‑99 (MQ=11)
                         ttAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCATGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggc                                               >  1:2641407/1‑98 (MQ=11)
                         ttAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTTAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggc                                               >  1:6303581/1‑98 (MQ=11)
                         ttAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACCCAGGAGAGAACGAAGTTTTGACTACAgggc                                               >  1:531210/1‑98 (MQ=11)
                          tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct                                              >  1:1070083/1‑97 (MQ=11)
                          tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct                                              >  1:2359160/1‑97 (MQ=11)
                          tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct                                              >  1:2566322/1‑97 (MQ=11)
                          tAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggca                                              >  1:4383481/1‑97 (MQ=11)
                          tAGCCTTGGAAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggct                                              >  1:33654/1‑97 (MQ=11)
                           aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGGGAACGAAGTTTTGACTACAgggctg                                             >  1:2268379/1‑96 (MQ=11)
                           aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg                                             >  1:3726397/1‑96 (MQ=11)
                           aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAgggctg                                             >  1:2429999/1‑96 (MQ=11)
                           aGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGCGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAggggca                                             >  1:2423555/1‑96 (MQ=11)
                                      aTGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAGGGCTGTTAcctcctat                                  >  1:5285011/1‑98 (MQ=2)
                                           cctccCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAGGGCTGTTACCTCCTATggcgg                             >  1:2924857/1‑96 (MQ=2)
                                           cctccCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAGGGCTGTTACCTCCTATggcgg                             >  1:1439884/1‑96 (MQ=2)
                                              cccGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTACAGGGCTGTTACCTCCTATggcgggcc                          <  1:1450552/100‑8 (MQ=2)
                                                                      tgtTCCGTCGTACTCAGGATCCACTCAAGAGAGACAACATTTTCGACTACAGGATTATTACCTTCTTTGATTCATCTTTCCAGATGATTCGTCTAATgtc  <  1:305466/100‑1 (MQ=32)
                                                                                                 |                                                                        
TTCACTATCGGTCACTAGAGAGTATTTAGCCTTAGGAGATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAAGAGAGACAACATTTTCGACTACAGGATTATTACCTTCTTTGATTCATCTTTCCAGATGATTCGTCTAATGTC  >  NC_007793/2000197‑2000366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑