Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	349,857	G→A	intergenic (+304/‑282)	SAUSA300_RS01585 → / → SAUSA300_RS01590	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	349,857	0	G	A	100.0%	38.6 / NA	16	intergenic (+304/‑282)	SAUSA300_RS01585/SAUSA300_RS01590	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/0);  total (16/0)

ATCAAGATTATTATAATTGTATTACATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTGCTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAGTAGGAGTTTTAGGG  >  NC_007793/349767‑349916                                                                                           |                                                            aTCACGATTATTATAATTGTATTACATATGGGTGCTGGCAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATta                                                    >  1:977808/1‑100 (MQ=17)   cAAGATTATTATAATTGTATTACATATCGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATtata                                                  >  1:6498231/1‑100 (MQ=255)                       tACATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGaaa                              >  1:6623540/1‑100 (MQ=255)                       caCATATAGGTGCTGGTAAATTTTATTTACGAAATGTCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGaaa                              >  1:2100523/2‑100 (MQ=255)                            ataGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaa                         >  1:4381241/1‑100 (MQ=255)                            ataGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaa                         >  1:5065415/1‑100 (MQ=255)                             taGATGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaa                        >  1:6619435/1‑100 (MQ=255)                             accctGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaa                        >  1:4452340/5‑100 (MQ=255)                              aGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaaa                       >  1:1769051/1‑100 (MQ=255)                                  gCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAaataat                   >  1:6051473/1‑100 (MQ=255)                                    tGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATgc                 >  1:2750450/1‑99 (MQ=255)                                     ggTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAg                >  1:1145181/1‑100 (MQ=255)                                     ggTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAg                >  1:5695337/1‑100 (MQ=255)                                     ggTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAAGGAg                >  1:2060445/1‑100 (MQ=255)                                     ggTAAATTTTATTTACGAAATGGC‑AAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGact               >  1:5959962/1‑98 (MQ=255)                                                   aCGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAGTAGGAGtagatcgg  >  1:4440581/1‑93 (MQ=255)                                                                                           |                                                            ATCAAGATTATTATAATTGTATTACATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTGCTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAGTAGGAGTTTTAGGG  >  NC_007793/349767‑349916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑