Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,980,852	T→A	intergenic (‑200/+156)	SAUSA300_RS09835 ← / ← SAUSA300_RS09840	transcriptional regulator/DNA‑binding response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,980,852	0	T	A	100.0%	117.1 / NA	41	intergenic (‑200/+156)	SAUSA300_RS09835/SAUSA300_RS09840	transcriptional regulator/DNA‑binding response regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/41);  total (0/41)

AAAAGAGTCAATTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAATTTTTATGCATAATCACTAGATTTGATAAAACTTACACTTTTATAATGATATCAAATTTACAAAGAAAACTGTATAATAAACCCTATCGCTATA  >  NC_007793/1980761‑1980945                                                                                            |                                                                                              aaaaGAGTCAATTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGc                                                                                       <  1:4055614/100‑1 (MQ=255) aaaaGAGTCAATTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGc                                                                                       <  1:4687995/100‑1 (MQ=255)    agagTCAATTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATa                                                                                    <  1:3946454/100‑1 (MQ=255)    agagTCAATTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATa                                                                                    <  1:3852848/100‑1 (MQ=255)    agagTCAATTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATa                                                                                    <  1:4590429/100‑1 (MQ=255)          aaTTTCATATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:4921648/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:5144566/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:4813367/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:5499609/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:5585441/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:4468660/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:436318/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:1160420/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:6574998/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:3970661/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:908924/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:4186684/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:3388537/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:1278780/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:1418869/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:1484043/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:1660645/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:1834301/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:188009/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:3271557/100‑1 (MQ=255)          aaTTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAATAAATGAAGTAAAATTTTATGCATAATCACt                                                                              <  1:4948024/100‑1 (MQ=255)            tttCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACTAg                                                                            <  1:4421843/100‑1 (MQ=255)            tttCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACTAg                                                                            <  1:3770228/100‑1 (MQ=255)            tttCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACTAg                                                                            <  1:5707053/100‑1 (MQ=255)            tttCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACTAg                                                                            <  1:4264549/100‑1 (MQ=255)                   tttGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACTAGATTTGAt                                                                     <  1:5306377/100‑1 (MQ=255)                               tttGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACTAGATTTGATAAAACTTacaca                                                         <  1:1627530/100‑2 (MQ=255)                                ttGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACTAGATTTGATAAAACTTACACtg                                                        <  1:857167/100‑2 (MQ=255)                                   aTCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACTAGATTTGATAAAACTTacacactag                                                     <  1:4148572/100‑6 (MQ=255)                                          caacaaGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACTAGATTTGATAAAACTTACACTTTTATAATGat                                              <  1:2350175/100‑1 (MQ=255)                                                                 taATGAAGCGAAAGTAAATGAAGTAAAATTTTATGCATAATCACTAGATTTGATAAAACTTACACTTTTATAATGATATCAAATTTACAAAGAAAACTGt                       <  1:1323661/100‑1 (MQ=255)                                                                           aaaGTAAATGAAGTAAAATTTTATGCATAATCACTAGATTTGATAAAACTTACACTTTTATAATGATATCAAATTTACAAAGAAAACTGTATAATAAAcc             <  1:6111735/100‑1 (MQ=255)                                                                             aGTAAATGAAGTAAAATTTTATGCATAATCACTAGATTTGATAAAACTTACACTTTTATAATGATATCAAATTTACAAAGAAAACTGTATAATAAACCCt           <  1:3144646/100‑1 (MQ=255)                                                                               tAAATGAAGTAAAATTTTATGCATAATCACTAGATTTGATAAAACTTACACTTTTATAATGATATCAAATTTACAAAGAAAACTGTATAATAAACCCTag         <  1:6612234/100‑2 (MQ=255)                                                                                     aaGTAAAATTTTATGCATAATCACTAGATTTGATAAAACTTACACTTTTATAATGATATCAAATTTACAAAGAAAACTGTATAATAAACCCTATCGCtat   <  1:1435023/100‑1 (MQ=255)                                                                                      aGTAAAATTTTATGCATAATCACTAGATTTGATAAAACTTACACTTTTATAATGATATCAAATTTACAAAGAAATCTGTATAATAAACCCTATCGCtata  <  1:4744913/100‑1 (MQ=255)                                                                                            |                                                                                              AAAAGAGTCAATTTCACATTTGTATTAAATTTTGATCAAGTCAACAAGAGTTAAGTTTACTTTATAATGAAGCGAAAGTAAATGAAGTAAATTTTTATGCATAATCACTAGATTTGATAAAACTTACACTTTTATAATGATATCAAATTTACAAAGAAAACTGTATAATAAACCCTATCGCTATA  >  NC_007793/1980761‑1980945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑