Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,977,282	G→A	A140V (GCA→GTA)	SAUSA300_RS09815 ←	DNA repair exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,977,282	0	G	A	100.0%	84.6 / NA	31	A140V (GCA→GTA)	SAUSA300_RS09815	DNA repair exonuclease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/24);  total (7/24)

AGATTTACTATACGTACCATGCAAGACACCAATATGTATGCCTTTTTGGCCTTGACTTGATGGATATTCATCAATCTTGTTCTCATAACTTGCTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCAACTTTATTTGAAAATACAGAAA  >  NC_007793/1977191‑1977375                                                                                            |                                                                                              aGATTTACTATACGTACCATGCAAGACACCAATATGTATGCCTTTTTGGCCTTGACTTGATGGATATTCATCAATCATGTTCTCATAACTTACTCTAttt                                                                                       <  1:3226057/100‑1 (MQ=255)         tataCGTACCATGCAAGACACCAATATGTATGCCTTTTTGGCCTTGACTTGATGGATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACt                                                                               <  1:5580426/100‑1 (MQ=255)                  cATGCAAGACACCAATATGTATGCCTTTTTGGCCTTGACTTGATGGATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTg                                                                      >  1:4704474/1‑100 (MQ=255)                   aTGCAAGACACCAATATGTATGCCTTTTTGGCCTTGACTTGATGGATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGa                                                                     >  1:6067990/1‑100 (MQ=255)                           tacCAATATGTATGCCTTTTTTGCCTTGACTTGATGGATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAAt                                                             >  1:6151774/2‑100 (MQ=255)                                    gtatgCCTTTTTGGCCTTGACTTGATGGATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCAcc                                                    <  1:5695292/100‑1 (MQ=255)                                    cttccgatctttGGCCTTGACTTGATGGATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCAcc                                                    <  1:4180494/91‑1 (MQ=255)                                               tGGCCTTGACTTGATGGATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTAACACCAGAtcggaaga                                         >  1:2447519/1‑93 (MQ=255)                                                     tgacttgaTGGATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCtg                                   <  1:919853/100‑2 (MQ=255)                                                        tctgaTGGCTATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCAt                                <  1:3689366/98‑1 (MQ=255)                                                        cttgaTGGATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCag                                <  1:5155652/100‑2 (MQ=255)                                                        cttgaTGGATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAAACCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCAt                                <  1:4990509/100‑1 (MQ=255)                                                               gATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACggagt                         <  1:2176381/100‑5 (MQ=255)                                                               gATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGtctc                         <  1:136262/100‑1 (MQ=255)                                                               gATATTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGtctc                         <  1:4540863/100‑1 (MQ=255)                                                               gATATTCAGCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACgggtt                         <  1:6179162/100‑5 (MQ=255)                                                                atatTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCa                        <  1:1146160/100‑1 (MQ=255)                                                                atatTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATCTAGTAATTGCTTCATACGTCTCa                        <  1:6228177/100‑1 (MQ=255)                                                                ataaTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGtctcc                        <  1:2873531/100‑2 (MQ=255)                                                                 tatTCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCag                       <  1:3198710/100‑2 (MQ=255)                                                                 gctcttccgaTCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCat                       <  1:592784/91‑2 (MQ=255)                                                                   ttCATCAATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCaagt                     <  1:2142643/100‑3 (MQ=255)                                                                    tcatcaATCTTGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCAACtg                    <  1:5335503/100‑2 (MQ=255)                                                                              tGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCAACTTTATTTGaaaa          >  1:3146144/1‑100 (MQ=255)                                                                              tGTTCTCATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCAACTTTATTTGaaaa          >  1:2590026/1‑100 (MQ=255)                                                                                 tctcATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCGCCAGATTTAGTAATTGCTTCATACGTCTCAACTTTATTTGAAAATAc       <  1:2207518/100‑1 (MQ=255)                                                                                 tctcATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCAACTTTATTTGAAAATAc       <  1:2335223/100‑1 (MQ=255)                                                                                 tctcATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCAACTTTATTTGAAAATAc       <  1:6524677/100‑1 (MQ=255)                                                                                 tcccATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCAACTTTATTTGAAAATAc       <  1:1830908/97‑1 (MQ=255)                                                                                    cATAACTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCAACTTTATTTGAAAATACAGa    >  1:6525984/1‑100 (MQ=255)                                                                                      gatcTTACTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCAACTTTATTTGAAAATACAGaag  <  1:1900696/97‑2 (MQ=255)                                                                                            |                                                                                              AGATTTACTATACGTACCATGCAAGACACCAATATGTATGCCTTTTTGGCCTTGACTTGATGGATATTCATCAATCTTGTTCTCATAACTTGCTCTATTTTCATAACTAAATCCGTGAATATAAATTGTTTCACCAGATTTAGTAATTGCTTCATACGTCTCAACTTTATTTGAAAATACAGAAA  >  NC_007793/1977191‑1977375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑