Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,959,504	T→A	intergenic (+127/+1116)	SAUSA300_RS09695 → / ← SAUSA300_RS09700	hypothetical protein/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,959,504	0	T	A	100.0%	14.1 / NA	7	intergenic (+127/+1116)	SAUSA300_RS09695/SAUSA300_RS09700	hypothetical protein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (2/5);  total (2/5)

ATGAAACGAATTTGAAAATTTCGAAACAGCTTAAGATAGCAAATTGAATAGCCTTATTGATAATGCAGAATCGTCTACACTTAGTTGAACTAATTCTATGAGAATAGATATTGTTAAACTATTTGGGTAGGCGATTTTATTTTGACAAGAGTCAGAAGATTATTTAGTTCAT  >  NC_007793/1959414‑1959585                                                                                           |                                                                                  aTGAAACGAATTTGAAAATTTCGAAACAGCTTAAGATAGCAAATTGAATAGCCTTATTGATAATGCAGAATCGTCTACACTTAGTTGAACAAATTCTATg                                                                          <  1:5282432/100‑1 (MQ=255)              gAAAATTTCGAAACAGCTTAAGATAGCAAATTGAATAGCCTTATTGATAATGCAGAATCGTCTACACTTAGTTGAACAAATTCTATgagatcggaagagc                                                             >  1:5752688/1‑90 (MQ=38)                                                aTAGCCTTATTGATAATGCAGAATCGTCTACACTTAGTTGAACAAATTCTATGAGAATAGATATTGTTAAACTATTTGGGTAGGCGATTTTATTTTGACa                           <  1:6467774/100‑1 (MQ=255)                                                  aGCCTTATTGATAATGCAGAATCGTCTACACTTAGTTGAACAAATTCTATGAGAATAGATATTGTTAAACTATTTGGGTAGGCGATTTTATTTTGACAag                         >  1:197399/1‑100 (MQ=255)                                                      ttATTGATAATGCAGAATCGTCTACACTTAGTTGAACAAATTCTATGAGAATAGATATTGTTAAACTATTTGGGTAGGCGATTTTATTTTGACAAGAGTc                     <  1:1328931/100‑1 (MQ=255)                                                                         gTCTACACTTAGTTGAACAAATTCTATGAGAATAGATATTGTTAAACTATTTGGGTAGGCGATTTTATTTTGACAAGAGTCAGAAGATTATTTAGTTCAt  <  1:2423359/100‑1 (MQ=255)                                                                         gTCTACACTTAGTTGAACAAATTCTATGAGAATAGATATTGTTAAACTATTTGGGTAGGCGATTTTATTTTGACAAGAGTCAGAAGATTATTTAGTTCAt  <  1:3668797/100‑1 (MQ=255)                                                                                           |                                                                                  ATGAAACGAATTTGAAAATTTCGAAACAGCTTAAGATAGCAAATTGAATAGCCTTATTGATAATGCAGAATCGTCTACACTTAGTTGAACTAATTCTATGAGAATAGATATTGTTAAACTATTTGGGTAGGCGATTTTATTTTGACAAGAGTCAGAAGATTATTTAGTTCAT  >  NC_007793/1959414‑1959585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑