Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,957,984	A→T	intergenic (‑745/‑138)	SAUSA300_RS09685 ← / → SAUSA300_RS09690	gamma‑hemolysin subunit A/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,957,984	0	A	T	100.0%	47.6 / NA	19	intergenic (‑745/‑138)	SAUSA300_RS09685/SAUSA300_RS09690	gamma‑hemolysin subunit A/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/19);  total (0/19)

ATATTTTATTTTTCAATACTTAATTTCAAAAATCCCCTATGAAAATGTTGATGAGCTACATCTTTAAATCTTATTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGCGTATGCATCTCACCTATC  >  NC_007793/1957912‑1958063                                                                         |                                                                                atatTTTATTTTTCAATACTTAATTTTAAAAATCCCCTATGAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCg                                                      <  1:3287675/100‑1 (MQ=255)                                 tCCCCTATGAAAATGTTGATGAGCTGCATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGtt                      <  1:4648123/100‑1 (MQ=255)                                  ccccTATGAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTg                     <  1:4297283/100‑1 (MQ=255)                                     ttgtGAAAATGTTGATGAGGTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTAGACACTTTTGGTTgtgt                  <  1:2002363/97‑1 (MQ=255)                                     ctgtGAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTgtgt                  <  1:4851761/97‑1 (MQ=255)                                     cTATGAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACATTTTTGGTTgtgt                  <  1:961675/100‑1 (MQ=255)                                     cTATGAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTgtgt                  <  1:809546/100‑1 (MQ=255)                                     cTATGAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTgtgt                  <  1:5608692/100‑1 (MQ=255)                                      tgtGAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGTGTa                 <  1:5428282/98‑1 (MQ=255)                                       aTGAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGTGTag                <  1:80432/100‑2 (MQ=255)                                         gAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGTGTATGc              <  1:1841803/100‑1 (MQ=255)                                         gAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGTGTATGc              <  1:5425413/100‑1 (MQ=255)                                         gAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGTGTATGc              <  1:5158480/100‑1 (MQ=255)                                         gAAAATGTTGATGAGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATACTGCGAGGTAGTAAATTGATATTAAACACTTTTGGTTGTGTATGc              <  1:4704981/100‑1 (MQ=255)                                                  gatgaGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGTGTATGCATCTCACCt     <  1:5091605/100‑1 (MQ=255)                                                  gatgaGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGTGTATGCATCTCACCt     <  1:6452079/100‑1 (MQ=255)                                                  gatgaGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGTGTATGCATCTCACCt     <  1:172521/100‑1 (MQ=255)                                                    tgaGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGTGTATGCATCTCACCTAt   <  1:6029960/100‑1 (MQ=255)                                                     gaGCTACATCTTTAAATCTTTTTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGTGTATGCATCTCACCTagg  <  1:256389/100‑3 (MQ=255)                                                                         |                                                                                ATATTTTATTTTTCAATACTTAATTTCAAAAATCCCCTATGAAAATGTTGATGAGCTACATCTTTAAATCTTATTATACATTGTATAAAATTATATTGCGAGGTAGTAAATTGATATTATACACTTTTGGTTGCGTATGCATCTCACCTATC  >  NC_007793/1957912‑1958063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑