Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,945,182	C→G	N183K (AAC→AAG)	SAUSA300_RS09630 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,945,182	0	C	G	100.0%	17.7 / NA	9	N183K (AAC→AAG)	SAUSA300_RS09630	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (1/8);  total (1/8)

CTTCTCCAGTCTCGGGTAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATA  >  NC_007793/1945106‑1945273                                                                             |                                                                                            ctcttccgatctcGGGTAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCGCAATTGATTAAACCtt                                                                      <  1:491382/91‑1 (MQ=255) ctcttccgatctcGGGCAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCtt                                                                      <  1:4911012/91‑1 (MQ=255)                 tAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCtt                                                      <  1:5607018/100‑1 (MQ=255)                                     cTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATtaataa                                  >  1:1434386/1‑100 (MQ=255)                                                            actaaACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAaccaca           <  1:2983429/100‑2 (MQ=255)                                                             ctaaACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCt          <  1:5794123/100‑1 (MQ=255)                                                               aaaCGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAACCATTAATAACTACCCCTTTCTTACAACCCACCTAg        <  1:4106309/100‑1 (MQ=255)                                                                aaCGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAgg       <  1:4676097/100‑2 (MQ=255)                                                                     ttCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCata  <  1:5738007/100‑1 (MQ=255)                                                                             |                                                                                            CTTCTCCAGTCTCGGGTAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCATA  >  NC_007793/1945106‑1945273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑