Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	345,030	A→G	Y106C (TAT→TGT)	SAUSA300_RS01550 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	345,030	0	A	G	100.0%	62.0 / NA	24	Y106C (TAT→TGT)	SAUSA300_RS01550	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/6);  total (18/6)

GCACAGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTATTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCTATTTATTATTA  >  NC_007793/344936‑345115                                                                                               |                                                                                      gCACAGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTg                                                                                  >  1:171007/1‑100 (MQ=255)    caGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAg                                                                               >  1:2267560/1‑100 (MQ=255)                       atGCTCGATTTTTAGTTGTTATAAATGTGTTAACTGCTATCACTGCAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGa                                                            >  1:3088440/1‑100 (MQ=255)                       atGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGa                                                            >  1:6211988/1‑100 (MQ=255)                            cGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGatttat                                                       >  1:2507426/1‑100 (MQ=255)                                          tataAATGTGTTAACTACTATCACTGTGGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTg                                         <  1:4003947/100‑1 (MQ=255)                                          tataAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTg                                         <  1:1215903/100‑1 (MQ=255)                                          tataAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTg                                         <  1:5408878/100‑1 (MQ=255)                                             aaaTGTGTTATCTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATg                                      <  1:4487043/100‑1 (MQ=255)                                             aaaGGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATg                                      <  1:847047/100‑1 (MQ=255)                                                 gtgtTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATggggt                                  <  1:5757772/100‑3 (MQ=255)                                                           ctaTCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAAt                        >  1:664715/1‑100 (MQ=255)                                                             aTCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCa                      >  1:2537667/1‑100 (MQ=255)                                                                   gtagtaTTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTa                >  1:3222792/1‑100 (MQ=255)                                                                          ttGCACTGTGTAATCTTTATTGTTTTGCCGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtattt         >  1:470785/1‑100 (MQ=255)                                                                          ttGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtattt         >  1:2587030/1‑100 (MQ=255)                                                                          ttGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtattt         >  1:5679402/1‑100 (MQ=255)                                                                           tGCCCTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCACTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtattta        >  1:3884264/1‑100 (MQ=255)                                                                           tGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtattta        >  1:1117124/1‑100 (MQ=255)                                                                            gCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtatttat       >  1:933992/1‑100 (MQ=255)                                                                            gCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtatttat       >  1:3919043/1‑100 (MQ=255)                                                                            gCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtatttat       >  1:3094453/1‑100 (MQ=255)                                                                              aCTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCTATttatta     >  1:2296060/1‑100 (MQ=255)                                                                                 gtgtAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGTTGGGTTCGATTTCAATCATAATTAGCTATttattatta  >  1:5612890/1‑100 (MQ=255)                                                                                               |                                                                                      GCACAGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTATTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCTATTTATTATTA  >  NC_007793/344936‑345115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑