Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	328,214	A→G	T84T (ACA→ACG)	SAUSA300_RS01475 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	328,214	0	A	G	100.0%	34.6 / NA	15	T84T (ACA→ACG)	SAUSA300_RS01475	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/0);  total (15/0)

TTATGGCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACACTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATTTACGTTTTTGGTG  >  NC_007793/328117‑328275                                                                                                  |                                                              ttATGGCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCt                                                             >  1:5017268/1‑100 (MQ=255)  tATGGCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCtt                                                            >  1:1554364/1‑100 (MQ=255)   ttGGCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTa                                                           >  1:2477447/2‑100 (MQ=255)     ggCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAAc                                                         >  1:5228453/1‑100 (MQ=255)     ggCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAAc                                                         >  1:677794/1‑100 (MQ=255)           tACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCGCGCTTAACGTTAtt                                                   >  1:4846337/1‑100 (MQ=255)                  gTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATACGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGat                                            >  1:6009653/1‑100 (MQ=255)                                gatgTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTg                              >  1:3817697/1‑100 (MQ=255)                                gatgTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTg                              >  1:5564555/1‑100 (MQ=255)                                   gTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCtt                           >  1:6379074/1‑100 (MQ=255)                                     acatacaAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCtttt                         >  1:495534/1‑100 (MQ=255)                                     acatacaAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATAAGAGGATAGATATCTCAAGCTTAACGTTATTGTGGGATATAATTTGCCATTGCtttt                         >  1:5865181/1‑100 (MQ=255)                                     acatacaAGAGAAAAATGAAAATTACAAAGTTG‑AAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCttttt                        >  1:1334593/1‑100 (MQ=255)                                        aacaAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGt                      >  1:5072406/2‑100 (MQ=255)                                                            tACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATTTACGTTTTTGatg  >  1:2056965/1‑100 (MQ=255)                                                                                                  |                                                              TTATGGCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACACTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATTTACGTTTTTGGTG  >  NC_007793/328117‑328275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑