Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,924,930	T→C	intergenic (+229/‑3)	SAUSA300_RS09515 → / → SAUSA300_RS09520	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,924,930	0	T	C	100.0%	41.6 / NA	18	intergenic (+229/‑3)	SAUSA300_RS09515/SAUSA300_RS09520	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/2);  total (16/2)

AATCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGTAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTAAATTATAAAACAAATCTT  >  NC_007793/1924840‑1925025                                                                                           |                                                                                                agcccccATAAAGAAAATAGTTGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGacac                                                                                        >  1:583289/7‑100 (MQ=255) aaTCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGacac                                                                                        >  1:6409447/1‑100 (MQ=255) aaTCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGacac                                                                                        >  1:6391535/1‑100 (MQ=255) aaTCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAAACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGacac                                                                                        >  1:1148062/1‑100 (MQ=255) aaTCAACATAAAGAAAATAGCAGTAAATCAAATGACACTAATAAAAAGACGCAACAACCTGACAACACTACACAGTCAAATACAGAAAAGCAAATGacac                                                                                        >  1:2004783/1‑100 (MQ=255)              aaaaTAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCgt                                                                           <  1:2682125/100‑2 (MQ=255)                   agtagtAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGata                                                                      >  1:5853334/1‑100 (MQ=255)                    gtagtaAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGGCAAATACAGAAAAGCAAATGACACCACAAGAAGGCGAAGatat                                                                     <  1:3692800/100‑1 (MQ=255)                                tacccacTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGa                                                         >  1:1677715/5‑100 (MQ=255)                                tacccacTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGa                                                         >  1:2994568/5‑100 (MQ=255)                                acgACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGa                                                         >  1:3842635/3‑100 (MQ=255)                                    cacTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTAc                                                     >  1:4564855/1‑100 (MQ=255)                                                   gCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAGAGCAagatcggaa                                      >  1:1595264/1‑94 (MQ=255)                                                             gACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATc                            >  1:2360860/1‑100 (MQ=255)                                                             gACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATc                            >  1:751781/1‑100 (MQ=255)                                                                     tACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTa                    >  1:549394/1‑100 (MQ=255)                                                                      acacAGACAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTTGAAACGTTTACAAAGCAAGAGGCGTTAATGAATATCAAACATTaa                   >  1:6242988/1‑100 (MQ=255)                                                                                       aaaGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTAAATTATAAAACAAATCtt  >  1:5876303/1‑100 (MQ=255)                                                                                           |                                                                                                AATCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGTAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTAAATTATAAAACAAATCTT  >  NC_007793/1924840‑1925025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑