Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,924,728	C→A	intergenic (+27/‑205)	SAUSA300_RS09515 → / → SAUSA300_RS09520	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,924,728	0	C	A	100.0%	38.0 / NA	16	intergenic (+27/‑205)	SAUSA300_RS09515/SAUSA300_RS09520	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/1);  total (15/1)

GCACCACCTAGCCAAAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTCTTTTACGGAAAAATTAATTATACATAATCAAATCAAGGAGATAAAAAAATGAAATTCAAAGCTATCGTTGCAATCACATTATCATTGT  >  NC_007793/1924642‑1924816                                                                                       |                                                                                         tgaccaccTAGCCATAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGaaaaa                                                                             >  1:1158324/3‑100 (MQ=255) gCACCACCTAGCCAAAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGaaaaa                                                                             >  1:3551644/1‑100 (MQ=255) gCACCACCTAGCCAAAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACCGaaaaa                                                                             >  1:2752942/1‑100 (MQ=255)    ccaccTAGCCAAAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGAAAaatta                                                                          >  1:6028568/1‑100 (MQ=255)     caccTAGCCAAAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGAAAaattaa                                                                         >  1:2592607/1‑100 (MQ=255)      accTAGCCAAAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGAAAaattaat                                                                        >  1:2253817/1‑100 (MQ=255)      accTAGCCAAAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGAAAaattaat                                                                        >  1:3515488/1‑100 (MQ=255)          aGCCAAAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGAAAAATTAATtata                                                                    >  1:3534581/1‑100 (MQ=255)          aGCCAAAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACCGAAAAATTAATtata                                                                    >  1:5806883/1‑100 (MQ=255)                   atTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGAAAAATTAATTATACATaatcaa                                                           >  1:3769438/1‑100 (MQ=255)                      cacaATGAAGATAGCACGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGAAAAATTAATTATACATaatcaaaac                                                        >  1:1593223/1‑98 (MQ=255)                       acaATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGAAAAATTAATTATACATaatcaaatca                                                       <  1:849844/100‑1 (MQ=255)                                 caGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGAAAAATTAATTATACATAATCAAATCAAGGAGAtcgg                                             >  1:4461311/2‑97 (MQ=255)                                  tgCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTATTTTACGGAAAAATTAATTATACATAATCAAATCAAGGAGAtcgga                                            >  1:2195037/2‑96 (MQ=255)                                  aGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGCAGCTCGGCTACCCTTATTTTACGGAAAAATTAATTATACATAATCAAATCAAGGAGAtcgga                                            >  1:3781678/1‑96 (MQ=255)                                                                            cGGCTACCCTTATTTTACGGAAAAATTAATTATACATAATCAAATCAAGGAGATAAAAAAATGAAATTCAAAGCTATCGTTGCAATCACATTATCATTGt  >  1:5792035/1‑100 (MQ=255)                                                                                       |                                                                                         GCACCACCTAGCCAAAATATTCACAATGAAGATAGCATGTACGACGCTTCAACAGAATAAAATTTCAGGGTAGCTCGGCTACCCTTCTTTTACGGAAAAATTAATTATACATAATCAAATCAAGGAGATAAAAAAATGAAATTCAAAGCTATCGTTGCAATCACATTATCATTGT  >  NC_007793/1924642‑1924816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑