Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	316,573	T→G	L56F (TTA→TTC)	SAUSA300_RS01415 ←	ribose transporter RbsU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	316,573	0	T	G	100.0%	18.0 / NA	9	L56F (TTA→TTC)	SAUSA300_RS01415	ribose transporter RbsU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/4);  total (5/4)

GAATCCCCATCCTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGTAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTACAG  >  NC_007793/316519‑316652                                                       |                                                                                tgaTCCCCATCCTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAAc                                    >  1:2729716/3‑100 (MQ=255)       ccATCCTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGAtcggaagagca                              >  1:2790614/1‑90 (MQ=255)            cTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGt                         >  1:5097240/1‑100 (MQ=255)               aaCCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGc                      >  1:3851656/2‑100 (MQ=255)                  cTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCagc                   <  1:2831647/100‑3 (MQ=255)                                 aTAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTAc    >  1:2484500/1‑100 (MQ=255)                                 aTAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTAc    <  1:380361/100‑1 (MQ=255)                                  tAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTAcg   <  1:2041330/100‑2 (MQ=255)                                   aGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTACat  <  1:2280805/100‑2 (MQ=255)                                                       |                                                                                GAATCCCCATCCTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGTAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTACAG  >  NC_007793/316519‑316652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑