Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,877,205	A→G	H228R (CAC→CGC)	SAUSA300_RS09290 →	NAD(FAD)‑utilizing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,877,205	0	A	G	100.0%	52.0 / NA	22	H228R (CAC→CGC)	SAUSA300_RS09290	NAD(FAD)‑utilizing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/9);  total (13/9)

CATCAGCTGAACCTTTCATCAAATCCAATCGTCTAAAAGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACACATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAACAAAAAAA  >  NC_007793/1877112‑1877301                                                                                              |                                                                                                 tctCAGCTGAACCTTTCATCAAATCCAATCGTCTAAAAGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAg                                                                                            >  1:462382/3‑100 (MQ=255)   tCAGCTGAACCTTTCATCAAATCCAATCGTCTAAAAGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGtc                                                                                          <  1:2448854/100‑1 (MQ=255)            ccTTTCAGCAAATCCAAACGTCTAAAAGGTTTAAGTTGAAAAGATGTTGAATTGTCAGGGCGTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATgg                                                                                 <  1:6524121/100‑1 (MQ=255)                           aaTCGTCTAAAAGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTc                                                                  >  1:3624320/1‑100 (MQ=255)                                   aaaaGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGt                                                          >  1:4527845/1‑100 (MQ=255)                                   aaaaGGATTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGt                                                          >  1:4786065/1‑100 (MQ=255)                                           taagtttaaAAGATGTTGAATTGTCAGTACTTAAGAAAAAGGGTAAAAAACGCATCAGTCGTCAAATGGATATGTTATTTACTCATTTTGGTATCAGTgg                                                  <  1:132025/100‑1 (MQ=255)                                                 taaAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGc                                            >  1:6262176/1‑100 (MQ=255)                                                  aaaaGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCt                                           >  1:5225221/1‑100 (MQ=255)                                                       aTGTTGAATTGCCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCAtt                                      <  1:4609514/100‑1 (MQ=255)                                                        tGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTa                                     <  1:3755572/100‑1 (MQ=255)                                                        tGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTa                                     <  1:4742716/100‑1 (MQ=255)                                                        tGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTa                                     <  1:1818646/100‑1 (MQ=255)                                                        tGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTAGCAGTGGTCCAGCTGCATTa                                     <  1:3755152/100‑1 (MQ=255)                                                        tGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGACATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTa                                     <  1:2436192/100‑1 (MQ=255)                                                         gTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTaa                                    >  1:4621372/1‑100 (MQ=255)                                                               ttGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTa                              >  1:304667/1‑100 (MQ=255)                                                                  tCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTc                           >  1:1641116/1‑100 (MQ=255)                                                                   cAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCa                          >  1:5797178/1‑100 (MQ=255)                                                                                         ccaaCGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGCCAGTTTGTTTATAAAGAACaaaaa    >  1:788832/3‑100 (MQ=255)                                                                                          caaCGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAACaaaaaa   >  1:1821826/2‑100 (MQ=255)                                                                                           aaCGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAACaaaaaaa  >  1:4328818/1‑100 (MQ=255)                                                                                              |                                                                                                 CATCAGCTGAACCTTTCATCAAATCCAATCGTCTAAAAGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACACATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAACAAAAAAA  >  NC_007793/1877112‑1877301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑