Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,838,956	A→G	V75A (GTA→GCA)	SAUSA300_RS09130 ←	PTS glucose transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,838,956	0	A	G	100.0%	183.0 / NA	70	V75A (GTA→GCA)	SAUSA300_RS09130	PTS glucose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/70);  total (0/70)

ATTTCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTACACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGTAGTAAAGA  >  NC_007793/1838860‑1839049                                                                                                 |                                                                                              atttCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTGGCTGCAAGCGCGACTgcag                                                                                            <  1:5075001/100‑2 (MQ=255)  tttCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTgcatt                                                                                           <  1:3707415/100‑3 (MQ=255)  tttCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCAcc                                                                                           <  1:3784231/100‑1 (MQ=255)  tttCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCAcc                                                                                           <  1:6627861/100‑1 (MQ=255)  gatctgAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCAcc                                                                                           <  1:1561430/95‑1 (MQ=255)   cttccgatcTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCGGCAAGCGCGACTGCACca                                                                                          <  1:3382567/92‑1 (MQ=255)    ttccgatcTTTCATCCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACcag                                                                                         <  1:5892975/93‑2 (MQ=255)    cgatctgCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACcat                                                                                         <  1:6080906/94‑1 (MQ=255)      atctgCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACcatca                                                                                       <  1:3656420/96‑1 (MQ=255)        gatcTTTCATCCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCAtt                                                                                     <  1:5430162/97‑1 (MQ=255)        atcttagcATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCAtt                                                                                     <  1:1879797/93‑1 (MQ=255)                  tCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAGCTGTAACTAAAAAATATCCGAACGTAGCTGCAAGCGCGACTGCTCCATCATTTTTCTTTGcc                                                                           <  1:5672209/100‑1 (MQ=255)                  tCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCTCCATCATTTTTCTTTGcc                                                                           <  1:3044926/100‑1 (MQ=255)                  tCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGcc                                                                           <  1:1479600/100‑1 (MQ=255)                  tCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGcc                                                                           <  1:5865801/100‑1 (MQ=255)                  tCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAACGCGACTGCACCATCATTTTTCTTTGcc                                                                           <  1:5370488/100‑1 (MQ=255)                  tCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACGAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGcc                                                                           <  1:1447333/100‑1 (MQ=255)                   ccTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCa                                                                          <  1:1094239/100‑1 (MQ=255)                   ccTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCa                                                                          <  1:28310/100‑1 (MQ=255)                    cTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAGCTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCAt                                                                         <  1:4451231/100‑1 (MQ=255)                    cTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCTCGACTGCACCATCATTTTTCTTTGCCag                                                                         <  1:6648069/100‑2 (MQ=255)                    cTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCag                                                                         <  1:1020925/100‑2 (MQ=255)                    cTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCAt                                                                         <  1:1047400/100‑1 (MQ=255)                     taataaAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCagg                                                                        <  1:6487808/100‑3 (MQ=255)                     taataaAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCA‑TTTTCTTTGCCAttg                                                                       <  1:3335208/100‑2 (MQ=255)                      aataaAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCAttg                                                                       <  1:1762166/100‑2 (MQ=255)                      aataaAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCAtag                                                                       <  1:2718391/100‑3 (MQ=255)                        taaAGGCGCTAATTTCATTGGTGATAATACAACTATAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCt                                                                     <  1:3828295/100‑1 (MQ=255)                          ctggCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtat                                                                   <  1:4891372/98‑1 (MQ=255)                          aaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtat                                                                   <  1:5227081/100‑1 (MQ=255)                          aaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtat                                                                   <  1:5520088/100‑1 (MQ=255)                          aaGGCGCTAATGTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtat                                                                   <  1:3334746/100‑1 (MQ=255)                              cgcTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCt                                                               <  1:3499367/100‑1 (MQ=255)                              cgcTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCt                                                               <  1:3866662/100‑1 (MQ=255)                                cTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTac                                                             <  1:3936407/100‑1 (MQ=255)                                cTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTac                                                             <  1:4021420/100‑1 (MQ=255)                                  ttccgatctGGTGTTAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTacag                                                           <  1:3921236/92‑2 (MQ=255)                                  aaTTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTacca                                                           <  1:1229247/100‑3 (MQ=255)                                  aaTTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTacat                                                           <  1:3471303/100‑2 (MQ=255)                                  aaTTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTacag                                                           <  1:4394262/100‑2 (MQ=255)                                  aaTTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTacac                                                           <  1:3510240/100‑1 (MQ=255)                                    tttCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCa                                                         <  1:5047248/100‑1 (MQ=255)                                    tttCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCa                                                         <  1:817730/100‑1 (MQ=255)                                     ttCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCac                                                        <  1:3884963/100‑2 (MQ=255)                                     ttCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCaa                                                        <  1:1690134/100‑1 (MQ=255)                                     ttCATTGGTGATAAGACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCaa                                                        <  1:4904994/100‑1 (MQ=255)                                       cATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAAtt                                                      <  1:5375909/100‑1 (MQ=255)                                         ttGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGc                                                    <  1:1046514/100‑1 (MQ=255)                                         ttGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGc                                                    <  1:6421428/100‑1 (MQ=255)                                         ttGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGc                                                    <  1:536960/100‑1 (MQ=255)                                          tGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCa                                                   <  1:2431365/100‑1 (MQ=255)                                           ggTGATAATACAACTGTAACTAGAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCac                                                  <  1:1947328/100‑2 (MQ=255)                                           ggTGATAATACAACGGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCCCCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCaa                                                  <  1:4802748/100‑1 (MQ=255)                                                                aaaaaaTATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTa                             <  1:1269665/100‑1 (MQ=255)                                                                aaaaaaTATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTa                             <  1:503377/100‑1 (MQ=255)                                                                aaaaaaTATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTa                             <  1:3511036/100‑1 (MQ=255)                                                                aaaaaaTATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTa                             <  1:2707016/100‑1 (MQ=255)                                                                aaaaaaTATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTa                             <  1:5591087/100‑1 (MQ=255)                                                                       atCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCgt                      <  1:2553858/100‑1 (MQ=255)                                                                         ccTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATCCCTAATTGCTCTAAAATCgtat                    <  1:4666430/100‑2 (MQ=255)                                                                              cGTAGCTGCAAGCGTGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGtacct               <  1:4957297/100‑1 (MQ=255)                                                                              cGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGtacct               <  1:2326683/100‑1 (MQ=255)                                                                              cGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGtacct               <  1:778408/100‑1 (MQ=255)                                                                              cGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGAAGtacct               <  1:5683842/100‑1 (MQ=255)                                                                                tAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGtacctaa             <  1:1830780/100‑2 (MQ=255)                                                                                  gCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGtacctaccc           <  1:572149/100‑2 (MQ=255)                                                                                       aaGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCgtagta      <  1:2498431/100‑1 (MQ=255)                                                                                       aaGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCgtagta      <  1:287702/100‑1 (MQ=255)                                                                                       aaGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCgtagta      <  1:3247731/100‑1 (MQ=255)                                                                                        aGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGTAGTaa     <  1:6063671/100‑1 (MQ=255)                                                                                        aGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGTAGTaa     <  1:2910577/100‑1 (MQ=255)                                                                                           gcgACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGTAGTaaggt  <  1:3543920/100‑4 (MQ=255)                                                                                                 |                                                                                              ATTTCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTACACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGTAGTAAAGA  >  NC_007793/1838860‑1839049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑