Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,745,180	T→C	I399M (ATA→ATG)	SAUSA300_RS08675 ←	single‑stranded DNA exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,745,180	0	T	C	100.0%	242.2 / NA	86	I399M (ATA→ATG)	SAUSA300_RS08675	single‑stranded DNA exonuclease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/85);  total (1/85)

TTCAGCTAACGATTCTATATTTTCAATATCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGTATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGGCAAAGC  >  NC_007793/1745084‑1745272                                                                                                 |                                                                                             ttCAGCTAACGATTCTATATTTTCAATATCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCAtt                                                                                           <  1:3002900/100‑1 (MQ=255)  tCAGCTAACGATTCTATATTTTCAATATCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCAttt                                                                                          >  1:3444838/1‑100 (MQ=255)         aCGATTCTATATTTTCAATACCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACag                                                                                   <  1:5679673/100‑2 (MQ=255)                  tatTTTCAATATCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTg                                                                          <  1:1864326/100‑1 (MQ=255)                     tttCAATATCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATc                                                                       <  1:495680/100‑1 (MQ=255)                     tttCAATATCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATc                                                                       <  1:3405377/100‑1 (MQ=255)                     tttCAATATCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATc                                                                       <  1:2212915/100‑1 (MQ=255)                        cAATATCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAAt                                                                    <  1:2981134/100‑1 (MQ=255)                          atatCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTg                                                                  <  1:1162694/100‑1 (MQ=255)                             tCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAAtgcaag                                                               <  1:2879746/100‑6 (MQ=255)                                 tCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGAGCTGGc                                                           <  1:2303565/100‑1 (MQ=255)                                   gTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCgg                                                         <  1:554192/100‑1 (MQ=255)                                   gTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCgg                                                         <  1:3851231/100‑1 (MQ=255)                                         ccTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCtt                                                   <  1:5552285/100‑1 (MQ=255)                                             cAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGc                                               <  1:51743/100‑1 (MQ=255)                                             cAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGc                                               <  1:5445493/100‑1 (MQ=255)                                             cAGCCATATGATGGCCGCCAAATTAAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGGCACTTGATCAATTGATCTGGCGGAGCCTTCTGc                                               <  1:1716623/100‑1 (MQ=255)                                               gCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCAt                                             <  1:4441521/100‑1 (MQ=255)                                                ccATATGATGGCCGTCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:4832798/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAGTTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:3503846/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGGTAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:1481131/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGGTCTGGCGGAGCCTTTTGCATg                                            <  1:3250311/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:3465140/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:916102/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:355644/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:6609291/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:4326122/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:4340654/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:4540421/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:4669383/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:492885/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:1119963/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:5018075/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:5267273/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:5335523/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:3384932/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:2623049/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:2925740/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:2458860/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:1861010/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:1823063/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:1805199/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:1668429/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACAGCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:1070813/100‑1 (MQ=255)                                                ccATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGAAAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATg                                            <  1:3556385/100‑1 (MQ=255)                                                 cATATGATGGCCGCTAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGa                                           <  1:5004894/100‑1 (MQ=255)                                                 cATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATgt                                           <  1:4048236/100‑2 (MQ=255)                                                 cATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATgg                                           <  1:6135866/100‑2 (MQ=255)                                                 cATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATgg                                           <  1:339596/100‑2 (MQ=255)                                                 cATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATgc                                           <  1:4573775/100‑2 (MQ=255)                                                 cATATGATGGCCGCCAAATTTAGCGACTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATgt                                           <  1:329382/100‑2 (MQ=255)                                                  atatGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAATCATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGAt                                          <  1:6234379/100‑1 (MQ=255)                                                  atatGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGctatca                                          <  1:864545/100‑6 (MQ=255)                                                  atatGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGag                                          <  1:1433654/100‑2 (MQ=255)                                                  atatGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCACTTCAAACATCGACACTGGATCAATTGATCTGGCGGAGCCTTTTGCATgca                                          <  1:2743748/100‑3 (MQ=255)                                                      gatgGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTg                                      <  1:2688926/100‑1 (MQ=255)                                                            cgccAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCt                                <  1:977220/100‑1 (MQ=255)                                                              ccAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTAt                              <  1:4619951/100‑1 (MQ=255)                                                              ccAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTAt                              <  1:3926996/100‑1 (MQ=255)                                                               cAAATTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATc                             <  1:4395300/100‑1 (MQ=255)                                                                aaaTTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGTGCCTTTTGCATGATTTTGCTCCCTAtgt                            <  1:4256769/100‑3 (MQ=255)                                                                aaaTTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGGGCCTTTTGCATGATTTTGCTCCCTATCa                            <  1:31201/100‑1 (MQ=255)                                                                aaaTTTAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGACTTTTGCATGATTTTGCTCCCTATCa                            <  1:4821682/100‑1 (MQ=255)                                                                     tAGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATAtt                       <  1:3231567/100‑1 (MQ=255)                                                                      aGCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATAttt                      <  1:1875268/100‑1 (MQ=255)                                                                       gCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTa                     <  1:3547417/100‑1 (MQ=255)                                                                       gCGATTAACTCTTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTa                     <  1:1504086/100‑1 (MQ=255)                                                                                tctTGATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAAtt            <  1:4056458/100‑2 (MQ=255)                                                                                 cgatctgtgCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGt           <  1:4441191/95‑1 (MQ=255)                                                                                    gATGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTgg        <  1:5219172/100‑1 (MQ=255)                                                                                     ctgtgCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgc       <  1:4012695/99‑1 (MQ=255)                                                                                     aTGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTgggt       <  1:1385695/100‑2 (MQ=255)                                                                                     aTGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgc       <  1:6333973/100‑1 (MQ=255)                                                                                     aTGTGCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgc       <  1:588302/100‑1 (MQ=255)                                                                                      tgtgCTGGTAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgca      <  1:4195604/100‑1 (MQ=255)                                                                                      tgtgCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgca      <  1:6610494/100‑1 (MQ=255)                                                                                      tgtgCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgca      <  1:833922/100‑1 (MQ=255)                                                                                      tgtgCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgca      <  1:3486338/100‑1 (MQ=255)                                                                                      tgtgCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgca      <  1:3916170/100‑1 (MQ=255)                                                                                      tgtgCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgca      <  1:4181066/100‑1 (MQ=255)                                                                                      tgtgCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgca      <  1:2165500/100‑1 (MQ=255)                                                                                      tctgCTGTTAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgca      <  1:2321545/98‑1 (MQ=255)                                                                                      cgatcTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgca      <  1:3625034/96‑1 (MQ=255)                                                                                       gtgCTGATAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgcaa     <  1:4402882/100‑1 (MQ=255)                                                                                       ctgttggtAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgcaa     <  1:2717434/93‑1 (MQ=255)                                                                                          cTGGTAGCATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGgcaaagc  <  1:2896957/100‑1 (MQ=255)                                                                                                 |                                                                                             TTCAGCTAACGATTCTATATTTTCAATATCCATCGTCATACCTGCAGCCATATGATGGCCGCCAAATTTAGCGATTAACTCTTGATGTGCTGATAGTATTTCAAACATCGACACTTGATCAATTGATCTGGCGGAGCCTTTTGCATGATTTTGCTCCCTATCAATATTTAAAATTAATGTTGGCAAAGC  >  NC_007793/1745084‑1745272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑