Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,725,858	G→A	T550I (ACA→ATA)	SAUSA300_RS08590 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,725,858	0	G	A	100.0%	191.2 / NA	66	T550I (ACA→ATA)	SAUSA300_RS08590	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/61);  total (5/61)

TACCGGCATTTCCTTTATACATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTGTTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGATGTGCTA  >  NC_007793/1725763‑1725951                                                                                                |                                                                                              tACCGGCATTTCCTTTATACATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTgg                                                                                           <  1:1321189/100‑1 (MQ=255) tACCGGCATTTCCTTTATACATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTgg                                                                                           >  1:6610331/1‑100 (MQ=255)  ctcGGCATTTCCTTTATACATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGt                                                                                          <  1:1719454/98‑1 (MQ=255)           tCCTTTATACGTTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGttt                                                                                 <  1:6308009/100‑1 (MQ=255)           tCCTTTATACATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGctt                                                                                 >  1:2662288/1‑100 (MQ=255)              tttATACATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGcc                                                                              <  1:439296/100‑1 (MQ=255)               ttATACATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGccc                                                                             <  1:5947215/100‑2 (MQ=255)               ttATACATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCt                                                                             <  1:5158452/100‑1 (MQ=255)                tataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAGCTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTg                                                                            <  1:2286207/100‑1 (MQ=255)                tataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTTCCTTCTCAACAACTATTGGTATTTGGTTTGCCTg                                                                            <  1:6171600/100‑1 (MQ=255)                tataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTg                                                                            <  1:769273/100‑1 (MQ=255)                tataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTg                                                                            <  1:5123759/100‑1 (MQ=255)                tataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTg                                                                            <  1:6582585/100‑1 (MQ=255)                tataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTg                                                                            <  1:6653272/100‑1 (MQ=255)                tataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTg                                                                            <  1:1588978/100‑1 (MQ=255)                 ataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTTCCTTCTCAACAACTATTGGTATTTGGTTTGCCTGa                                                                           <  1:3726858/100‑1 (MQ=255)                 ataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTgt                                                                           <  1:6106144/100‑2 (MQ=255)                 ataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTgt                                                                           <  1:6008691/100‑2 (MQ=255)                 ataCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTgt                                                                           <  1:3908279/100‑2 (MQ=255)                 acaCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAACACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGa                                                                           >  1:5041792/3‑100 (MQ=255)                  taCATTGGTGTAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGag                                                                          <  1:2156618/100‑2 (MQ=255)                  taCATTGGTGCAAGTACTTGAATATCAGCCATAGTATCCCCTTTATTAACAGCACTAGTAAATACCTTCTCAACAACTATTGGTATTTGGTTTGCCTgtg                                                                          <  1:4811954/100‑3 (MQ=255)                  taCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCGCTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGAc                                                                          <  1:2804026/100‑1 (MQ=255)                  taCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAGCTACCTTCGCAACAACTATTGGTATTCGGTTTGCCTGAc                                                                          <  1:3704703/100‑1 (MQ=255)                  taCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTgta                                                                          <  1:4617455/100‑3 (MQ=255)                  taCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGaa                                                                          <  1:3548229/100‑2 (MQ=255)                  taCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGAc                                                                          <  1:6520271/100‑1 (MQ=255)                  taCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGAc                                                                          <  1:5193632/100‑1 (MQ=255)                  taCATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGAc                                                                          <  1:6348500/100‑1 (MQ=255)                      ttGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGtt                                                                      <  1:559125/100‑1 (MQ=255)                      ttGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGtt                                                                      <  1:1676097/100‑1 (MQ=255)                      ttGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGtt                                                                      <  1:1719146/100‑1 (MQ=255)                       tGGTGCAAGCACTTGAATATCAGCCATAGTATCCCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTta                                                                     <  1:2794260/100‑1 (MQ=255)                       tGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTta                                                                     <  1:4519182/100‑1 (MQ=255)                       tGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTta                                                                     <  1:4622160/100‑1 (MQ=255)                         gTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTtaat                                                                   <  1:6152595/100‑1 (MQ=255)                                   ttGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTa                                                         <  1:4602289/100‑1 (MQ=255)                                        tatCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATg                                                    <  1:1483783/100‑1 (MQ=255)                                         atCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGa                                                   <  1:177976/100‑1 (MQ=255)                                         atCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGa                                                   <  1:310387/100‑1 (MQ=255)                                          tCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCAtcac                                                  <  1:4720382/100‑4 (MQ=255)                                          tCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGaa                                                  <  1:3367089/100‑1 (MQ=255)                                                  aGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCtgt                                          <  1:4473987/100‑1 (MQ=255)                                                  aGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCtgt                                          <  1:4102296/100‑1 (MQ=255)                                                   gTATACCCTTTATTAACAGCACTAGGAACTACCTTCTCAACACCTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCtttg                                         <  1:6246514/100‑1 (MQ=255)                                                    tataCCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTgtgt                                        <  1:3179753/100‑1 (MQ=255)                                                    tataCCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTgtgt                                        <  1:3384295/100‑1 (MQ=255)                                                    tataCCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTgtgt                                        <  1:6032584/100‑1 (MQ=255)                                                           tttATTAACAGCACTAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCa                                 <  1:2744138/100‑1 (MQ=255)                                                                         tccgatcTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATCAACCTTCTATCATGAAAACGCTGTGTAAAATCAATTTTCTGACCCag                   <  1:1137735/94‑2 (MQ=255)                                                                         tAGTAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCaa                   <  1:4621636/100‑1 (MQ=255)                                                                           cgatcTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCaagt                 <  1:5193894/96‑3 (MQ=255)                                                                            tAACTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACtt                <  1:4739330/100‑1 (MQ=255)                                                                              aCTACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGAccccgggact              <  1:6572541/100‑8 (MQ=255)                                                                                tACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAATACTGATATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCAtt            <  1:6220395/100‑1 (MQ=255)                                                                                tACCTTCTCAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCAtt            <  1:5769584/100‑1 (MQ=255)                                                                                     tctcAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGAtg       <  1:4200873/100‑1 (MQ=255)                                                                                     tctcAACAACTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGAtg       >  1:1058491/1‑100 (MQ=255)                                                                                        caacaaCTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACTCTGTGTAATATCATTTTTCTGACCCAACTTCATTCGATGTGc    >  1:5280660/1‑100 (MQ=255)                                                                                        caacaaCTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGATGTGc    <  1:3079395/100‑1 (MQ=255)                                                                                         aacaacTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGATGTGCt   <  1:2926020/100‑1 (MQ=255)                                                                                          acaacTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGATGTGCTa  <  1:5833198/100‑1 (MQ=255)                                                                                          acaacTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGATGTGCTa  <  1:2310511/100‑1 (MQ=255)                                                                                          acaacTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGATGTGCTa  <  1:5094563/100‑1 (MQ=255)                                                                                          acaacTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGATGTGCTa  <  1:1078288/100‑1 (MQ=255)                                                                                          acaacTATTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGACGTGCTa  <  1:3716369/100‑1 (MQ=255)                                                                                                |                                                                                              TACCGGCATTTCCTTTATACATTGGTGCAAGCACTTGAATATCAGCCATAGTATACCCTTTATTAACAGCACTAGTAACTACCTTCTCAACAACTGTTGGTATTTGGTTTGCCTGACAGTTAATAAAACTTCTATCATGAAAACGCTGTGTAATATCAATTTTCTGACCCAACTTCATTCGATGTGCTA  >  NC_007793/1725763‑1725951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑