Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,699,824	A→C	V203G (GTT→GGT)	SAUSA300_RS08435 ←	competence protein ComEC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,699,824	0	A	C	100.0%	13.0 / NA	7	V203G (GTT→GGT)	SAUSA300_RS08435	competence protein ComEC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/2);  total (5/2)

GACAAATAAAGGTAAATTTAATCGTTTTAAAGGTTGGTAAATTAAAAATACAATTGCAGCTATATGCGAGCCACTAACTGCCAGCAAATGATATATAACTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAGTAATCAATGCCATAATACGA  >  NC_007793/1699727‑1699898                                                                                                  |                                                                           gACAAATAAAGGTAAATTTAATCGTTTTAAAGGTTGGTAAATTAAAAATACAATTGCAGCTATATGCGAGCCACTAACTGCCAGCAAATGATATATACCt                                                                          <  1:1557387/100‑1 (MQ=255) gACAAATAAAGGTAAATTTAATCGTTTTAAAGGTTGGTAAATTAAAAATACAATTGCAGCTATATGCGAGCCACTAACTGCCAGCAAATGATATATACCt                                                                          >  1:4010885/1‑100 (MQ=255)                                   tGGTAAATTAAAAATACAATTGCAGCTATATGCGAGCCACTAACTGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTAAATTGCTCATTaa                                        >  1:3315133/1‑100 (MQ=255)                                                        ccAGCTATATGCGAGCCACTAAATGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAgatc                   >  1:4448848/2‑97 (MQ=255)                                                           tcTATATGCGAGCCACTAACTGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACATCACCAgatcgga                >  1:1055657/2‑94 (MQ=255)                                                                      cttccgatcTGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAGTAATCAATGCCataata     <  1:6022024/92‑1 (MQ=255)                                                                         aCTAACTGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAGTAATCAATGCCatacagatc  >  1:5295650/1‑94 (MQ=255)                                                                                                  |                                                                           GACAAATAAAGGTAAATTTAATCGTTTTAAAGGTTGGTAAATTAAAAATACAATTGCAGCTATATGCGAGCCACTAACTGCCAGCAAATGATATATAACTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAGTAATCAATGCCATAATACGA  >  NC_007793/1699727‑1699898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑