Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,693,739	T→C	T145A (ACA→GCA)	SAUSA300_RS08410 ←	coproporphyrinogen III oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,693,739	0	T	C	100.0%	20.8 / NA	10	T145A (ACA→GCA)	SAUSA300_RS08410	coproporphyrinogen III oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/9);  total (1/9)

CCATATCTAAAGCTAGATCTAAACTTTGTTCAAAATCTTCAATCGTCTGTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGTGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTAAAACAGACAATAACTCCGGCTTGA  >  NC_007793/1693643‑1693819                                                                                                 |                                                                                 ccATATCTAAAGCTAGATCTAAACTTTGTTCAAAATCTTCAATCGTCTGTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTgcgta                                                                               <  1:3411607/100‑2 (MQ=255)    gatCTCAAGTTAGATCTAAACTTTGTTCAAAATCTTCAATCGTCTGTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGttttt                                                                            <  1:4567514/99‑1 (MQ=255)                   cTAAACTTTGTTCAAAATCTTCAATCGTCTGTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGa                                                             <  1:3370136/100‑1 (MQ=255)                     aaaCTTTGTTCAAAATCTTCAATCGTCTGTTTCGGTAAATGATACATTAAGTCTAAACTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAg                                                           >  1:4145259/1‑100 (MQ=255)                                               cTGTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGtt                                 <  1:3566820/100‑1 (MQ=255)                                               cTGTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGtt                                 <  1:6019868/100‑1 (MQ=255)                                               cTGTTTCGGTAAATGATACATTAAATCGAAACTGATTGATTTAATACCGGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGtt                                 <  1:569278/100‑1 (MQ=255)                                                tGTTTCAGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGttt                                <  1:5301596/100‑2 (MQ=255)                                                 gTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTca                               <  1:911391/100‑2 (MQ=255)                                                                            aaCTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTAAAACAGACAATAACTCCGGCtt    <  1:974384/100‑1 (MQ=255)                                                                              cTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTAAAACAGACAATAACTCCGGCTTGa  <  1:41475/100‑1 (MQ=255)                                                                                                 |                                                                                 CCATATCTAAAGCTAGATCTAAACTTTGTTCAAAATCTTCAATCGTCTGTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGTGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTAAAACAGACAATAACTCCGGCTTGA  >  NC_007793/1693643‑1693819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑