Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,663,728	T→C	I30V (ATA→GTA)	SAUSA300_RS08255 ←	penicillin‑binding protein 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,663,728	0	T	C	100.0%	147.3 / NA	54	I30V (ATA→GTA)	SAUSA300_RS08255	penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/52);  total (2/52)

GTAATGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATATAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAACTACCTA  >  NC_007793/1663631‑1663824                                                                                                  |                                                                                                 gTAATGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACaa                                                                                                >  1:2622739/1‑100 (MQ=255)  tAATGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATTACCTAAACGTAGTACTAGTACTGCAAAAATAAATACaag                                                                                               <  1:6602285/100‑2 (MQ=255)   aaTGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATc                                                                                              <  1:4525478/100‑1 (MQ=255)     tGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcac                                                                                            <  1:1327349/100‑1 (MQ=255)     tGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcac                                                                                            <  1:6322509/100‑1 (MQ=255)     tGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcac                                                                                            <  1:6299931/100‑1 (MQ=255)     tGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcac                                                                                            <  1:1947393/100‑1 (MQ=255)      gTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcagg                                                                                           <  1:5995009/100‑3 (MQ=255)       ttttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcacag                                                                                          <  1:3762202/100‑2 (MQ=255)       ttttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCacac                                                                                          <  1:2179585/100‑1 (MQ=255)       ttttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCacac                                                                                          <  1:5365826/100‑1 (MQ=255)       ttttCATCATTTTTTATAATTTGTTTATAATGTAAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcacaa                                                                                          <  1:3708397/100‑2 (MQ=255)        tttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACAcc                                                                                         <  1:1179274/100‑1 (MQ=255)        tttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACAcc                                                                                         <  1:1146767/100‑1 (MQ=255)        tttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACAcc                                                                                         <  1:5343164/100‑1 (MQ=255)        tttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACAcc                                                                                         <  1:5463935/100‑1 (MQ=255)         ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                        <  1:5235249/100‑1 (MQ=255)         ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                        <  1:4460955/100‑1 (MQ=255)         ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                        <  1:5773775/100‑1 (MQ=255)         ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                        <  1:3974959/100‑1 (MQ=255)         ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                        <  1:3603122/100‑1 (MQ=255)         ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                        <  1:3062551/100‑1 (MQ=255)         ttCATCATTTTTTATAATTTGTATATAAGGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATTCAATCACACCa                                                                                        <  1:6653984/100‑1 (MQ=255)          tcatcaTTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTGAATAACCTAAGCGTAGTGCTAGTACTGCAAAAATAAATACAATCACACCaa                                                                                       <  1:4481195/100‑1 (MQ=255)          tcatcaTTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAGACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCaa                                                                                       <  1:5106258/100‑1 (MQ=255)          tcatcaTTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACAccta                                                                                       <  1:3296371/100‑3 (MQ=255)          tcatcaTTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCaa                                                                                       <  1:1483525/100‑1 (MQ=255)          tcatcaTTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCaa                                                                                       <  1:2344548/100‑1 (MQ=255)           catcatTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACAcctta                                                                                      <  1:5520455/100‑4 (MQ=255)           catcatTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCagg                                                                                      <  1:3450905/100‑3 (MQ=255)           catcatTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCaag                                                                                      <  1:3114788/100‑2 (MQ=255)            atcatTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAat                                                                                     <  1:2742933/100‑1 (MQ=255)              catTTTTTATAACTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAatat                                                                                   <  1:226020/100‑1 (MQ=255)               atTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAatatg                                                                                  <  1:681983/100‑2 (MQ=255)                    ttATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGtt                                                                             <  1:2822901/100‑1 (MQ=255)                    ttATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGtt                                                                             <  1:3132804/100‑1 (MQ=255)                     tataATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTa                                                                            <  1:4574281/100‑1 (MQ=255)                     tataATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTa                                                                            <  1:1266403/100‑1 (MQ=255)                            tGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTctct                                                                     <  1:4843811/100‑1 (MQ=255)                               ttATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCGAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGt                                                                  <  1:4323421/100‑1 (MQ=255)                                tataATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGta                                                                 <  1:6260338/100‑2 (MQ=255)                                      gtgAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAGAATCAATCCAATCACACCAAATATAAAGTTAATTCCCTTGTTAATTGt                                                           <  1:5235838/100‑1 (MQ=255)                                      gtgAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGt                                                           <  1:2615382/100‑1 (MQ=255)                                                    aTTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGaggg                                             <  1:264356/100‑4 (MQ=255)                                                         tAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCGTGTTAATTGTATTTTGAACGATTtcatca                                        <  1:3280511/100‑1 (MQ=255)                                                             tAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTg                                    <  1:3524023/100‑1 (MQ=255)                                                                   aaaCGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGAttttt                              >  1:3722023/1‑100 (MQ=255)                                                                        tagtactagtacTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCtttt                         <  1:5370483/100‑1 (MQ=255)                                                                                             aaataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTCTTTAACAAAActacc    <  1:4617779/100‑1 (MQ=255)                                                                                              aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCGTCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct   <  1:3871092/100‑1 (MQ=255)                                                                                              aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct   <  1:4078286/100‑1 (MQ=255)                                                                                              aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct   <  1:2437586/100‑1 (MQ=255)                                                                                              aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct   <  1:6623254/100‑1 (MQ=255)                                                                                              aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct   <  1:423173/100‑1 (MQ=255)                                                                                               atacAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAGCAAAActaccta  <  1:675516/100‑1 (MQ=255)                                                                                               atacAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActaccta  <  1:1760907/100‑1 (MQ=255)                                                                                                  |                                                                                                 GTAATGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATATAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAACTACCTA  >  NC_007793/1663631‑1663824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑