Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,644,224	C→T	intergenic (‑201/+194)	SAUSA300_RS08130 ← / ← SAUSA300_RS08135	hypothetical protein/elongation factor P

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,644,224	0	C	T	100.0%	59.8 / NA	23	intergenic (‑201/+194)	SAUSA300_RS08130/SAUSA300_RS08135	hypothetical protein/elongation factor P
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/22);  total (1/22)

ATTAAAAATTTTATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTCTACTCATGTCTATGTTTTTTCACTACCAATTAGACGATTCGTTTTATATTTTAAAAATTATTTTAAAAATAAAAAGCCTCATATCAGCATAT  >  NC_007793/1644129‑1644316                                                                                                |                                                                                             aTTAAAAATTTTATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACt                                                                                          <  1:6411056/100‑1 (MQ=255)  ttAAAAATTTTATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACta                                                                                         <  1:3072176/100‑2 (MQ=255)       aaTTTTATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATgag                                                                                    <  1:3364681/100‑3 (MQ=255)        aTTTTATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCt                                                                                   <  1:5126904/100‑1 (MQ=255)        aTTTTATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCt                                                                                   <  1:6429621/100‑1 (MQ=255)        aTTTTATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCt                                                                                   <  1:5575475/100‑1 (MQ=255)        aTTTTATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCAGGTCt                                                                                   <  1:6022358/100‑1 (MQ=255)         ttttATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCTa                                                                                  <  1:460005/100‑1 (MQ=255)         ttttATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCTa                                                                                  <  1:4099319/100‑1 (MQ=255)          tttATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCTac                                                                                 <  1:482146/100‑2 (MQ=255)           ttATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATgggggca                                                                                <  1:6531447/100‑7 (MQ=255)                         aaaCATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCTATGTTTTTTCACTACCa                                                                  <  1:4684831/100‑1 (MQ=255)                         aaaCATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCTATGTTTTTTCACTACCa                                                                  <  1:4175217/100‑1 (MQ=255)                          aaCATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCTATGTTTTTTCACTACCaa                                                                 <  1:6011949/100‑1 (MQ=255)                            cATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCTATGTTTTTTCACTACCAAtt                                                               <  1:88638/100‑1 (MQ=255)                            cATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCTATGTTTTTTCACTACCAAtt                                                               <  1:781041/100‑1 (MQ=255)                            cATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCTATGTTTTTTCACTACCAAtt                                                               <  1:6210709/100‑1 (MQ=255)                                                              tATCCTCAACAATATGATATGTAGATATATACCTTTACTCATGTCTATGTTTTTTCACTACCAATTAGACGATTCGTTTTAtattttaaaaattatttta                             >  1:5920028/1‑100 (MQ=255)                                                                             gatatgTAGATATATACCTTTACTCATGTCTATGTTTTTTCACTACCAATTAGACGATTCGTTTTATATTTTAAAAATTATTTTAAAAATAAAAAGCCta              <  1:5543705/100‑2 (MQ=255)                                                                               tttgTAGATATATACCTTTACTCATGACTATGTTTTTTCACTACCAAATAGACGGTTCGTTTTATATTTTAAAAATTATTTTATAAATAAAAAGCCTCat            <  1:5901682/98‑1 (MQ=255)                                                                                       tatataCCTTTACTCATGTCTATGTTTTTTCACTACCAATTAGACGGTTCGTTTTATATTTTAAAAATTATTTTAAAAATAAAAAGCCTCATATCAGcgt    <  1:3202020/100‑3 (MQ=255)                                                                                        atataCCTTTACTCATGTCTATGTTTTTTCACTACCAATTAGACGATTCGTTTTATGTTTT‑AAAATTATTTTAAAAATAAAAAGCCTCATATCAGCatat  <  1:5381841/100‑1 (MQ=255)                                                                                        atataCCTTTACTCATGTCTATGTTTTTTCACTACCAATTAGACGATTCGTTTTATATTTTAAAAATTATTTTAAAAATAAAAAGCCTCATATCAGCatc   <  1:5722303/100‑2 (MQ=255)                                                                                                |                                                                                             ATTAAAAATTTTATGCATTCAGTTAAACATATCAATGTTATTTTTATTAGCTATTATGTTTTATCCTCAACAATATGATATGTAGATATATACCTCTACTCATGTCTATGTTTTTTCACTACCAATTAGACGATTCGTTTTATATTTTAAAAATTATTTTAAAAATAAAAAGCCTCATATCAGCATAT  >  NC_007793/1644129‑1644316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑