Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,635,412	G→A	D184D (GAC→GAT)	SAUSA300_RS08085 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,635,412	0	G	A	100.0%	26.8 / NA	11	D184D (GAC→GAT)	SAUSA300_RS08085	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/11);  total (0/11)

ATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATATTACCATCAATAGAAGATGTACTATTCATAATTATATTATTTAAGTCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGCATTCGCAAGTATACCAACACCAAAAC  >  NC_007793/1635322‑1635479                                                                                           |                                                                    aTTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTAcat                                                            <  1:3591504/100‑3 (MQ=255)        atatGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATAttgt                                                     <  1:1941741/100‑1 (MQ=255)           tGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATAttgtttg                                                  <  1:3623654/100‑1 (MQ=255)                 aTACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCag                                            <  1:4167436/100‑2 (MQ=255)                     cTGAATTCTCATGAATATAGTCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTACATTATTCAAATCCTTACCAATATTGTTTGTAccccatgt                                        <  1:5916690/100‑7 (MQ=255)                                 gAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGc                            <  1:735487/100‑1 (MQ=255)                                  aaTATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTgga                           <  1:2128882/100‑3 (MQ=255)                                   atatAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGcta                          <  1:5916169/100‑3 (MQ=255)                                    tataATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGcctg                         <  1:1362075/100‑4 (MQ=255)                                    tataATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGccag                         <  1:2760336/100‑4 (MQ=255)                                                           gaagaTGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGCATTCGCAAGTATACCAACACCaaacc  <  1:4623867/100‑3 (MQ=255)                                                                                           |                                                                    ATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATATTACCATCAATAGAAGATGTACTATTCATAATTATATTATTTAAGTCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGCATTCGCAAGTATACCAACACCAAAAC  >  NC_007793/1635322‑1635479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑