Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,635,367	A→G	N199N (AAT→AAC)	SAUSA300_RS08085 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,635,367	0	A	G	100.0%	21.0 / NA	11	N199N (AAT→AAC)	SAUSA300_RS08085	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/11);  total (0/11)

TCATTACTAAATATGTTCTATATATCGTCATATCAAATAATTGATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATATTACCATCAATAGAAGATGTACTATTCATAATTATATTATTTAAGTCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGCATT  >  NC_007793/1635279‑1635456                                                                                         |                                                                                          tCATTACTAAATATGTTCTATATATCGTCATATCAAATAATTGATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCAAt                                                                                <  1:6151904/100‑1 (MQ=255)                                            aTTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTAcat                                     <  1:3591504/100‑3 (MQ=255)                                                   atatGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATAttgt                              <  1:1941741/100‑1 (MQ=255)                                                      tGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATAttgtttg                           <  1:3623654/100‑1 (MQ=255)                                                            aTACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCag                     <  1:4167436/100‑2 (MQ=255)                                                                cTGAATTCTCATGAATATAGTCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTACATTATTCAAATCCTTACCAATATTGTTTGTAccccatgt                 <  1:5916690/100‑7 (MQ=255)                                                                            gAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGc     <  1:735487/100‑1 (MQ=255)                                                                             aaTATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTgga    <  1:2128882/100‑3 (MQ=255)                                                                              atatAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGcta   <  1:5916169/100‑3 (MQ=255)                                                                               tataATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGcctg  <  1:1362075/100‑4 (MQ=255)                                                                               tataATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGccag  <  1:2760336/100‑4 (MQ=255)                                                                                         |                                                                                          TCATTACTAAATATGTTCTATATATCGTCATATCAAATAATTGATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATATTACCATCAATAGAAGATGTACTATTCATAATTATATTATTTAAGTCCTTACCAATATTGTTTGTACCACGTATAATATTACCTGCATT  >  NC_007793/1635279‑1635456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑