Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,634,467	G→A	N499N (AAC→AAT)	SAUSA300_RS08085 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,634,467	0	G	A	100.0%	25.9 / NA	11	N499N (AAC→AAT)	SAUSA300_RS08085	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

CTCGTACTTGATGTTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATTATTTGCATTTGATTCAACAGAAGCAGATTTTACATCGTTACCATTCATTGACTCTTGTTGTTCTCTGCGCTTATTACCATTT  >  NC_007793/1634379‑1634512                                                                                         |                                              ctcGTACTTGATGCTCTTGCTGTGATTCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACcattcat                                    >  1:3796756/1‑100 (MQ=255)         tGATGCTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCtt                            <  1:1068615/100‑1 (MQ=255)           aTGCTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCttgt                          <  1:6327842/100‑1 (MQ=255)             gctCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCttgttg                        <  1:403712/98‑1 (MQ=255)                 ttGCTGTGATGCTTTTTGTGGTTGGTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTtct                    <  1:5682088/100‑1 (MQ=255)                  tGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTtctc                   >  1:3066687/1‑100 (MQ=255)                             tttttGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTtcgccgggta        <  1:4590563/100‑7 (MQ=255)                              ttttGAGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCttattag       >  1:3015157/1‑99 (MQ=255)                                 tgtgGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTCGTTCTCTTCGCTTATTACCAt    >  1:254079/1‑100 (MQ=255)                                  gtgGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCAtt   <  1:3720701/100‑1 (MQ=255)                                   tgGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCAtgg  <  1:5075742/100‑3 (MQ=255)                                                                                         |                                              CTCGTACTTGATGTTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATTATTTGCATTTGATTCAACAGAAGCAGATTTTACATCGTTACCATTCATTGACTCTTGTTGTTCTCTGCGCTTATTACCATTT  >  NC_007793/1634379‑1634512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑