Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,632,345	T→G	E28A (GAA→GCA)	SAUSA300_RS08070 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,632,345	0	T	G	100.0%	39.5 / NA	17	E28A (GAA→GCA)	SAUSA300_RS08070	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/15);  total (2/15)

ACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCATGATAAATTCAC  >  NC_007793/1632309‑1632438                                     |                                                                                              gactcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTa                                <  1:1086139/91‑1 (MQ=255) gactcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTa                                >  1:1602665/10‑100 (MQ=255)     cactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATAc                            >  1:5142556/6‑100 (MQ=255)        tcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACaaa                         <  1:5608163/98‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc                       <  1:6059737/100‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc                       <  1:1909989/100‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc                       <  1:5343077/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa                      <  1:1700747/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa                      <  1:4145220/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCGTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa                      <  1:2392353/100‑1 (MQ=255)                   ctttCAAAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCa              <  1:1774560/99‑1 (MQ=255)                       cagaatttaaaaCTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCATGAt          <  1:4796026/90‑1 (MQ=255)                        agaatttaaaaCTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCATGATa         <  1:1168318/91‑1 (MQ=255)                         gaatttaaaaCTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCACGATat        <  1:221871/92‑2 (MQ=255)                             ttaaaaCTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCATGATaagcat    <  1:6156679/96‑5 (MQ=255)                             ttaaaaCTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCATGATAAATTc    <  1:5269953/96‑1 (MQ=255)                               aaaaCTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCATGATAAATTCAc  <  1:1265793/98‑1 (MQ=255)                                     |                                                                                              ACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCATGATAAATTCAC  >  NC_007793/1632309‑1632438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑