Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,632,335	C→A	K31N (AAG→AAT)	SAUSA300_RS08070 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,632,335	0	C	A	100.0%	26.3 / NA	11	K31N (AAG→AAT)	SAUSA300_RS08070	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (2/9);  total (2/9)

ACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCA  >  NC_007793/1632309‑1632426                           |                                                                                            gactcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTa                    <  1:1086139/91‑1 (MQ=255) gactcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTa                    >  1:1602665/10‑100 (MQ=255)     cactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATAc                >  1:5142556/6‑100 (MQ=255)        tcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACaaa             <  1:5608163/98‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc           <  1:1909989/100‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc           <  1:5343077/100‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc           <  1:6059737/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa          <  1:1700747/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa          <  1:4145220/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCGTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa          <  1:2392353/100‑1 (MQ=255)                   ctttCAAAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCa  <  1:1774560/99‑1 (MQ=255)                           |                                                                                            ACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCATCGCCTCA  >  NC_007793/1632309‑1632426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑