Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	291,848	A→G	H44R (CAT→CGT)	SAUSA300_RS01295 →	sorbitol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	291,848	0	A	G	100.0%	37.5 / NA	15	H44R (CAT→CGT)	SAUSA300_RS01295	sorbitol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/1);  total (14/1)

TGATAAAGAAGTTGCAACACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCATACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAAATCGTTGAAGTTGGAGCAGACGTTAA  >  NC_007793/291768‑291938                                                                                 |                                                                                           tGATAAAGAAGTTGCAACACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATtat                                                                         >  1:4190868/1‑100 (MQ=255)              gCAACACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTc                                                            >  1:4080404/1‑100 (MQ=255)                acggccGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCa                                                          >  1:5079966/5‑100 (MQ=255)                   cgatcTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTg                                                       <  1:2425905/96‑1 (MQ=255)                    ccGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTATTTTTCCAGTGa                                                      >  1:4810470/1‑100 (MQ=255)                                               gTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGaaa                           >  1:1521761/1‑100 (MQ=255)                                                     tATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGaagatcgga                     >  1:4432903/1‑93 (MQ=255)                                                     tATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAGATCggaa                     >  1:3180711/1‑97 (MQ=255)                                                     tATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAAATCGTTg                     >  1:2357685/1‑100 (MQ=255)                                                     tATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAAATCGTTg                     >  1:6354059/1‑100 (MQ=255)                                                     tATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATCTTCTGGTGAAATCGTTg                     >  1:314015/1‑100 (MQ=255)                                                      aTGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGTTGAGATCggaag                    >  1:4486469/1‑96 (MQ=255)                                                        gCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAGATCggaagag                  >  1:4105899/1‑94 (MQ=255)                                                        gCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGCCATGAATTTTCAGGTGaagatcggaaga                  >  1:2776653/1‑90 (MQ=255)                                                                        caGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAAATCGTTGAAGTTGGAGCAGatcggaa  >  1:1864097/1‑94 (MQ=255)                                                                                 |                                                                                           TGATAAAGAAGTTGCAACACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCATACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAAATCGTTGAAGTTGGAGCAGACGTTAA  >  NC_007793/291768‑291938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑