Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,632,325	C→T	E35K (GAA→AAA)	SAUSA300_RS08070 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,632,325	0	C	T	100.0%	22.4 / NA	10	E35K (GAA→AAA)	SAUSA300_RS08070	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/8);  total (2/8)

ACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCA  >  NC_007793/1632309‑1632418                 |                                                                                              gactcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTa            <  1:1086139/91‑1 (MQ=255) gactcactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTa            >  1:1602665/10‑100 (MQ=255)     cactcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATAc        >  1:5142556/6‑100 (MQ=255)        tcaTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACaaa     <  1:5608163/98‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc   <  1:1909989/100‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc   <  1:5343077/100‑1 (MQ=255)          aTACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAAcc   <  1:6059737/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa  <  1:1700747/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa  <  1:4145220/100‑1 (MQ=255)           tACTTTTTCTTTCAGAATTTAAAACTGCATCAATCTCGTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCa  <  1:2392353/100‑1 (MQ=255)                 |                                                                                              ACATCTTGAATACTTTCTTTTTCTAACTTTGTAACTTCATCAATTTCTTTATCATATTTCTGACCACATGCAGATAATAATATAACTAATCCTAAAATTAATACAAACCA  >  NC_007793/1632309‑1632418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑