Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 1,631,540 C→T K141K (AAG→AAA)  SAUSA300_RS08065 ← integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077931,631,5400CT98.1% 286.5 / ‑4.9 107K141K (AAG→AAA) SAUSA300_RS08065integrase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (5/100);  total (5/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 111 total aligned reads displayed.

TTCTTCACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTCTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACTTATATAATACCCTGTA  >  NC_007793/1631446‑1631636
                                                                                              |                                                                                                
ttcttcGCGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTGCGTCTAATTTGCCACGCGTTTCAGAAGACTTATTATATTTTTTGcc                                                                                             <  1:3417004/100‑1 (MQ=255)
 tcttcACGGTTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCt                                                                                            <  1:3696686/100‑1 (MQ=255)
 tcttcACGCTTATTAATACTTTTTGAGCCGATGCTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCt                                                                                            <  1:4272564/100‑1 (MQ=255)
 tcttcACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGGCTTATTATATTTTTTGCCt                                                                                            <  1:3496757/100‑1 (MQ=255)
 tcttcACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCt                                                                                            <  1:3081815/100‑1 (MQ=255)
  cttccgatctattaGTACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTg                                                                                           <  1:6264035/91‑1 (MQ=255)
  cttcACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTg                                                                                           <  1:2576507/100‑1 (MQ=255)
  cttcACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTg                                                                                           <  1:2466805/100‑1 (MQ=255)
   ttccgatctattgATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTgg                                                                                          <  1:3683618/92‑2 (MQ=255)
   ttcACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTgt                                                                                          <  1:5243648/100‑2 (MQ=255)
    tcACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCt                                                                                         <  1:4739464/100‑1 (MQ=255)
    acACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGccgcag                                                                                         >  1:4523282/2‑96 (MQ=255)
     ttccgatcttaGTACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTCGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGcgg                                                                                        <  1:4155623/92‑3 (MQ=255)
     cACTATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCtt                                                                                        <  1:2416044/100‑1 (MQ=255)
     cACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGccc                                                                                        >  1:227108/1‑98 (MQ=255)
     cACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCtt                                                                                        <  1:5625426/100‑1 (MQ=255)
     cACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCtt                                                                                        <  1:5325609/100‑1 (MQ=255)
     cACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCtt                                                                                        <  1:1596774/100‑1 (MQ=255)
     cACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAACACTTATTATATTTTTTGCCTGCtt                                                                                        <  1:2297762/100‑1 (MQ=255)
      aCGCTTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTg                                                                                       <  1:1879017/100‑1 (MQ=255)
      aCGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCtag                                                                                       <  1:1595928/100‑3 (MQ=255)
      aCGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTg                                                                                       <  1:4238037/100‑1 (MQ=255)
      aCGATTATTAATAC‑TTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAACTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTgc                                                                                      <  1:3760803/100‑2 (MQ=255)
       cGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGt                                                                                      <  1:3923363/100‑1 (MQ=255)
       cGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATGTTTTGCCTGCTTgg                                                                                      <  1:5664010/100‑2 (MQ=255)
       cGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTAAATTTTTTGCCTGCTTGt                                                                                      <  1:5538032/100‑1 (MQ=255)
        gATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGGCTTATTATATTTTTTGCCTGCTTGtc                                                                                     <  1:442050/100‑1 (MQ=255)
        gATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTgct                                                                                     <  1:5227992/100‑3 (MQ=255)
        gATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGtt                                                                                     <  1:1380377/100‑2 (MQ=255)
        gATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGtc                                                                                     <  1:1380983/100‑1 (MQ=255)
        gATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGtc                                                                                     <  1:2597191/100‑1 (MQ=255)
        gATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGATTGta                                                                                     <  1:79267/100‑2 (MQ=255)
         gttattaATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGtct                                                                                    <  1:4104724/99‑1 (MQ=255)
         attattAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGtct                                                                                    <  1:3290934/100‑1 (MQ=255)
          ttattaATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCGAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGtctc                                                                                   <  1:1258112/100‑1 (MQ=255)
           tattaATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGtctcg                                                                                  <  1:2677646/100‑2 (MQ=255)
            ggtaATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGCCTCag                                                                                 >  1:6541063/3‑99 (MQ=255)
                ttACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCa                                                                             <  1:834231/99‑1 (MQ=255)
                gtACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCa                                                                             <  1:5691548/99‑1 (MQ=255)
                aTACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCatatga                                                                             <  1:2059443/100‑3 (MQ=255)
                 tACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCaa                                                                            <  1:1722599/100‑2 (MQ=255)
                 tACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCAc                                                                            <  1:4718660/100‑1 (MQ=255)
                 tACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCCCATATCat                                                                            <  1:5766103/100‑2 (MQ=255)
                  aCTTTTTGAGCCGAGACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCAcc                                                                           <  1:4981069/100‑2 (MQ=255)
                      tttGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCtaa                                                                       <  1:5050509/100‑3 (MQ=255)
                      tttGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTa                                                                       <  1:1492373/100‑1 (MQ=255)
                      tttGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTa                                                                       <  1:3615468/100‑1 (MQ=255)
                      tttGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTa                                                                       <  1:3362537/100‑1 (MQ=255)
                        tGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTaag                                                                     <  1:2176477/100‑2 (MQ=255)
                        tGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAAc                                                                     <  1:4935274/100‑1 (MQ=255)
                           gCCGATACTTTATAAAACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATc                                                                  <  1:2245183/100‑1 (MQ=255)
                             cGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCat                                                                <  1:463389/100‑2 (MQ=255)
                                     tataACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTagg                                                        <  1:3763616/100‑3 (MQ=255)
                                     tataACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTAcc                                                        <  1:759263/100‑1 (MQ=255)
                                      ataACACGAGACCCTGCGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCa                                                       <  1:3567886/100‑1 (MQ=255)
                                      ataACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCa                                                       <  1:4078906/100‑1 (MQ=255)
                                      ataACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCa                                                       <  1:1399107/100‑1 (MQ=255)
                                      ataACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCa                                                       <  1:1474915/100‑1 (MQ=255)
                                      ataACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCa                                                       <  1:1886760/100‑1 (MQ=255)
                                      ataACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTACTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCa                                                       <  1:146867/100‑1 (MQ=255)
                                      ataACACGAGACCCCACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGGCTCATATCACGCTTAACATCAACTTTTACCa                                                       <  1:4839269/100‑1 (MQ=255)
                                         acacGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCagag                                                    <  1:1927963/100‑4 (MQ=255)
                                          cacGAGGCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATcc                                                   <  1:656802/100‑1 (MQ=255)
                                          cacGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATcc                                                   <  1:53012/100‑1 (MQ=255)
                                          cacGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATcc                                                   <  1:5694558/100‑1 (MQ=255)
                                          cacGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATcc                                                   <  1:3365605/100‑1 (MQ=255)
                                          cacGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATcc                                                   <  1:3040443/100‑1 (MQ=255)
                                          cacGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATACTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAAtgt                                                   <  1:2845553/100‑3 (MQ=255)
                                            cGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCag                                                 <  1:2792530/100‑2 (MQ=255)
                                            cGAGACCCGACGTCTAATTTGCCACGCTTTTCAGAAGACTAATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCAt                                                 <  1:5178811/100‑1 (MQ=255)
                                             gagaCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCAtt                                                <  1:2261398/100‑1 (MQ=255)
                                             gagaCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCAtt                                                <  1:5840910/100‑1 (MQ=255)
                                             gagaCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCAtt                                                <  1:367504/100‑1 (MQ=255)
                                             gagaCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCAtg                                                <  1:3891643/100‑2 (MQ=255)
                                             gagaCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCAtg                                                <  1:524650/100‑2 (MQ=255)
                                             gagaCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACCTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCAtg                                                <  1:144271/100‑2 (MQ=255)
                                             gagaCCCTACGTCTAATGTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCCCGCTTAACATCAACTTTTACCAATCCAtt                                                <  1:4389152/100‑1 (MQ=255)
                                              agaCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCagta                                               <  1:52302/100‑4 (MQ=255)
                                              agaCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCAtca                                               <  1:4811461/100‑3 (MQ=255)
                                              agaCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACGTTTACCAATCCAtgt                                               <  1:3189302/100‑3 (MQ=255)
                                               gaCCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGc                                              <  1:753994/100‑1 (MQ=255)
                                                 cccTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGa                                            <  1:4004881/100‑1 (MQ=255)
                                                  ccTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGaa                                           <  1:1869300/100‑1 (MQ=255)
                                                   cTACGTCTAATTTGTCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAat                                          <  1:4911311/100‑1 (MQ=255)
                                                   cTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCggta                                          <  1:3723112/100‑4 (MQ=255)
                                                   cTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAat                                          <  1:6434980/100‑1 (MQ=255)
                                                         cTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCt                                    <  1:2309797/100‑1 (MQ=255)
                                                              ttGCCACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCtatata                               >  1:669611/1‑100 (MQ=255)
                                                               tGCCACGCTTTTCAGAAGAGTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCtatatat                              >  1:2334164/1‑100 (MQ=255)
                                                                  cACGCTTTTCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATAtagt                           <  1:2397202/100‑1 (MQ=255)
                                                                        tttCAGAAGACTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATa                     <  1:6168205/100‑1 (MQ=255)
                                                                            agaagaCTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACtt                 <  1:5882921/100‑1 (MQ=255)
                                                                              aagaCTTATTATATTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACTtat               <  1:1515098/100‑1 (MQ=255)
                                                                                          tcttCTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACTTATATAATGCCCTGt   <  1:4447104/98‑1 (MQ=255)
                                                                                          cgatctcGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACTTATATAATGCCCTGt   <  1:1786143/97‑1 (MQ=255)
                                                                                          atTTTTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACTTATATAATGCCCTGt   <  1:1019520/100‑1 (MQ=255)
                                                                                          atTTTTTGCATGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACTTATATAATGCCCTGt   <  1:3506831/100‑1 (MQ=255)
                                                                                           ttttttGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACTTATATAATGCCCTGTa  <  1:852201/100‑1 (MQ=255)
                                                                                           ttttttGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACTTATATAATGCCCTGTa  <  1:512858/100‑1 (MQ=255)
                                                                                           tttCTTGCCCGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACTTATATAATGCCCTGTa  <  1:3408458/100‑1 (MQ=255)
                                                                                              |                                                                                                
TTCTTCACGATTATTAATACTTTTTGAGCCGATACTTTATAACACGAGACCCTACGTCTAATTTGCCACGCTTTTCAGAAGACTTATTATATTTCTTGCCTGCTTGTCTCATATCACGCTTAACATCAACTTTTACCAATCCATTGCGAATATATCTATATATAGTAGGATAACTTATATAATACCCTGTA  >  NC_007793/1631446‑1631636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑