Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,630,876	C→T	intergenic (‑289/+66)	SAUSA300_RS08055 ← / ← SAUSA300_RS08060	acetyl‑CoA carboxylase biotin carboxyl carrier protein subunit/integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,630,876	0	C	T	95.0%	44.8 / ‑6.0	20	intergenic (‑289/+66)	SAUSA300_RS08055/SAUSA300_RS08060	acetyl‑CoA carboxylase biotin carboxyl carrier protein subunit/integrase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (1/18);  minor base G (0/1);  total (1/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAAGCATTTTACTAGTAATATCTGTCAAAAACCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTACATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCTTAGATTAACCTATATTAATTGACGGGTTCGTGCCTC  >  NC_007793/1630799‑1630963                                                                              |                                                                                        aagaagCATTTTACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCgtcctgt                                                                   <  1:5448907/100‑1 (MQ=255)  agaagCAATTTACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTGTTAGATTAAGCAATTTAGATACAAAATTTATTCgtcctgag                                                                  <  1:2302210/100‑3 (MQ=255)    aagCATTTTCCTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCgtcctgtcca                                                                <  1:5072619/100‑2 (MQ=255)     agCATTTTACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCgtcctgtcctg                                                               <  1:2944321/100‑2 (MQ=255)     agCATTTTACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCtt                                                               <  1:6603651/100‑1 (MQ=255)     agCATTTTACTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCtt                                                               <  1:4106653/100‑1 (MQ=255)             aCTAGTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTc                                                       <  1:1401006/100‑1 (MQ=255)                 gTAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTg                                                   <  1:514123/100‑1 (MQ=255)                  tAATATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGt                                                  <  1:5441885/100‑1 (MQ=255)                   aaTATCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTa                                                 <  1:4323631/100‑1 (MQ=255)                    atatCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTgcct                                                <  1:4205105/100‑4 (MQ=255)                    atatCTGTCAAAAATCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTaa                                                <  1:6653061/100‑1 (MQ=255)                               aaaTCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCtt                                     <  1:1358662/100‑1 (MQ=255)                                                     gCTTCTTTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTGAACCATTCGCTTAGATTAACCTATATTAATTGAc               >  1:4904570/1‑100 (MQ=255)                                                      ctttttTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCTTAGATTAACCTATATTAATTGACg              <  1:540308/100‑1 (MQ=255)                                                      cttcttTTTAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCTTAGATTAACCTATATTAATTGACg              <  1:1459424/100‑1 (MQ=255)                                                         tcttccgatctATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCTTAGATTAACCTATATTAATTGACGGGt           <  1:1580498/90‑1 (MQ=255)                                                            tttAGATTATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCTTAGATTAACCTATATTAATTGACGGGTTCg        <  1:3743424/100‑1 (MQ=255)                                                                  ctttGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCTTAGATTAACCTATATTAATTGACGGGTTCGTGCCTc  <  1:136426/97‑1 (MQ=255)                                                                  ctATGCATTTTATATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCTTAGATTAACCTATATTAATTGACGGGTTCGTGCCTc  <  1:2319445/99‑1 (MQ=255)                                                                              |                                                                                        AAGAAGCATTTTACTAGTAATATCTGTCAAAAACCACAGATTTAAATAGTATGCTTCTTTTTAGATTATGCATTTTACATACAAAATTTATTCGTCCTGTCCTTCCGCGCTCATTGTAAACCATTCGCTTAGATTAACCTATATTAATTGACGGGTTCGTGCCTC  >  NC_007793/1630799‑1630963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑