Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,627,860	T→C	P22P (CCA→CCG)	SAUSA300_RS08035 ←	exodeoxyribonuclease 7 large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,627,860	0	T	C	100.0%	143.9 / NA	53	P22P (CCA→CCG)	SAUSA300_RS08035	exodeoxyribonuclease 7 large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/52);  total (1/52)

TAACGCTTTCTTTATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGTGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACATATAACATCACTC  >  NC_007793/1627763‑1627937                                                                                                  |                                                                              tcttccgatctttATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCgg                                                                             <  1:5214776/92‑1 (MQ=255) taACGCTTTCTTTATCTTTCACATTAAAGTATATATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCgg                                                                             <  1:2150959/100‑1 (MQ=255) taACGCTTTCTTTATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCGCCTTTAATCAATACAGATTGGAGATGCgg                                                                             <  1:5921474/100‑1 (MQ=255) taACGCTTTCTTTATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCgg                                                                             <  1:1192026/100‑1 (MQ=255) taACGCTTTCTTTATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCgg                                                                             <  1:4114313/100‑1 (MQ=255) taACGCTTTCTTTATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCgg                                                                             <  1:5297108/100‑1 (MQ=255) taACGCTTTCTTTATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCgg                                                                             <  1:3518075/100‑1 (MQ=255) taACGCTTTCTTTATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCAACTTTAATCAAGACAGATTGGAGATGCgg                                                                             <  1:1613056/100‑1 (MQ=255)       tttctttATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTg                                                                       <  1:5142339/100‑1 (MQ=255)       tttctttATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTg                                                                       <  1:3967806/100‑1 (MQ=255)        ttctttATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTgc                                                                      <  1:5751703/100‑2 (MQ=255)        cttccgaTCTTTCACGTTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCtggg                                                                      <  1:5409614/94‑4 (MQ=255)         ttccgaTCTTTTGCGTTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATccccaa                                                                     <  1:4903003/95‑6 (MQ=255)         tctttATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGAt                                                                     <  1:5393619/100‑1 (MQ=255)          tccgaTCTTTCACACTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGagg                                                                    <  1:4931149/96‑3 (MQ=255)          tccgaTCTTGCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATc                                                                    <  1:1129411/96‑1 (MQ=255)          tccgaTCTCTCACGTTAAAGTATAAATGACCACTTGAATGCTTTTCGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATc                                                                    <  1:5744107/96‑1 (MQ=255)          ctttATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATc                                                                    <  1:3741614/100‑1 (MQ=255)            ttATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGaagct                                                                  <  1:1267561/100‑5 (MQ=255)            cgaTCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCATTAACCAATACAGATTGGAGATGCGGATCTTgcacac                                                                  <  1:2807243/98‑6 (MQ=255)               tCTTTCACGTTAAAGTATAAATGACCACTGGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCaagga                                                               <  1:2926174/100‑4 (MQ=255)                  ttCACCTTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCaaggggcc                                                            <  1:5544853/100‑7 (MQ=255)                  ttCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCaggggctg                                                            <  1:5725440/100‑8 (MQ=255)                              tataAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAatatcac                                                <  1:3011875/100‑4 (MQ=255)                                   aTGACCGCTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGtt                                           <  1:6034230/100‑1 (MQ=255)                                          cTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTg                                    <  1:4066680/100‑1 (MQ=255)                                          cTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTg                                    <  1:5508221/100‑1 (MQ=255)                                           ttGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGa                                   <  1:5099491/100‑1 (MQ=255)                                           ttGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGAGCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGa                                   >  1:2855459/1‑100 (MQ=255)                                            tGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGaa                                  <  1:3182432/100‑1 (MQ=255)                                            tGAATGCTTTTTGAAATTTGAAAGCACACCTTTAATCAATGCAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGac                                  <  1:4595800/100‑2 (MQ=255)                                             gAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGtaa                                 <  1:3861058/100‑1 (MQ=255)                                             gAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGagc                                 <  1:4997377/100‑3 (MQ=255)                                                   tttttGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACtgg                           <  1:4242754/100‑3 (MQ=255)                                                    ttttGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAacacggtg                          <  1:4576542/100‑5 (MQ=255)                                                    ttttGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTaa                          <  1:4524953/100‑1 (MQ=255)                                                            tttgaaaGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTg                  <  1:5063585/100‑1 (MQ=255)                                                            tttgaaaGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTg                  <  1:2250419/100‑1 (MQ=255)                                                             ttgaaaGCTCGCCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTgg                 <  1:6591104/100‑2 (MQ=255)                                                              tgaatGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGAc                <  1:2607900/100‑1 (MQ=255)                                                              tgaaaGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTggg                <  1:1597809/100‑3 (MQ=255)                                                              tgaaaGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAACTGAAACACTTAAATAATCTGag                <  1:3282031/100‑2 (MQ=255)                                                                cgatcTCACCTCTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACat              <  1:4872385/96‑1 (MQ=255)                                                                aaaGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACat              <  1:3936895/100‑1 (MQ=255)                                                                 aaGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACata             <  1:5945527/100‑1 (MQ=255)                                                                  aGCTCACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACatat            <  1:3161125/100‑1 (MQ=255)                                                                   gcgcACCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACAtata           <  1:2221284/97‑1 (MQ=255)                                                                       gatcttAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACATATAACAt       <  1:4689901/96‑1 (MQ=255)                                                                       aCCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACATATAACag       <  1:1486526/100‑2 (MQ=255)                                                                       aCCTTTAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACATATAACAt       <  1:6501741/100‑1 (MQ=255)                                                                          tttAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACATATAACATCAc    <  1:1511074/100‑1 (MQ=255)                                                                           ttAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACATATAACATCACt   <  1:6209164/100‑1 (MQ=255)                                                                            tAATCAATACAGATTGGAGATGCGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACATATAACATCACTc  <  1:1943161/100‑1 (MQ=255)                                                                                                  |                                                                              TAACGCTTTCTTTATCTTTCACATTAAAGTATAAATGACCACTTGAATGCTTTTTGAAATTTGAAAGCTCACCTTTAATCAATACAGATTGGAGATGTGGATCTTGATCAAATTTATATTTAATATATTTCGTTAAAGCTGAAACACTTAAATAATCTGACATATAACATCACTC  >  NC_007793/1627763‑1627937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑