Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	275,084	G→A	P81S (CCA→TCA)	SAUSA300_RS01210 ←	long‑chain‑fatty‑acid‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	275,084	0	G	A	100.0%	10.3 / NA	6	P81S (CCA→TCA)	SAUSA300_RS01210	long‑chain‑fatty‑acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/3);  total (3/3)

TCAGCATAGAAGAGCAGTTTTAATTGTGCATCTTCCACAATGGCTGCAATTTCTTTTGGGTTAAGCCGCCAATTCAATGGTAAAAAAACCGCACCTGTTTTAAAACAAGCAAACAATAAATCTAATATTGCAATATCATTTGGCG  >  NC_007793/275005‑275149                                                                                |                                                                  tCAGCATAGAAGAGCAGTTTTAATTGTGCATCTTCCACAATGGCTGCAATTTCTTTTGGGTTAAGCCGCCAATTCAATGATAAAAAAACCGCACCTGttt                                               >  1:704232/1‑100 (MQ=255)  cAGCATAGAAGAGCAGTTTTAATTGTGCATCTTCCACAATGGCTGCAATTTCTTTTGGGTTAAGCCGCCAATTCAATGATAAAAAAACCGCACCTGtttt                                              >  1:783446/1‑100 (MQ=255)                aGTTTTAATTGTGCATCTTCCACAATGGCTGCAATTTCTTTTGGGTTAAGCCGCCAATTCAATGATAAAAAAACCGCACCTGTTTTAAAACAAGCAAACa                                <  1:2520839/100‑1 (MQ=255)                                 tccgatctaggCTGCAATTTCTTTTGGGTTAAGCCGCCAATTCAATGATAAAAAAACCGCACCTGTTTTAAAACAAGCAAACAATAAATCTAATATTGCa               <  1:1355095/91‑1 (MQ=255)                                      taaTGGCTGCAATTTCTTTTGGGTTAAGCCGCCAATTCAATGATAAAAAAACCGCACCTGTTTTAAAACAAGCAAACAATAAATCTAATATTGCAATATc          <  1:400961/99‑1 (MQ=255)                                              gCAATTTCTTTTGGGTTAAGCCGCCAATTCAATGATAAAAAAACCGCACCTGTTTTAAAACAAGCAAACAATAAATCTAATATTGCAATATCATTTGgcg  >  1:4912414/1‑100 (MQ=255)                                                                                |                                                                  TCAGCATAGAAGAGCAGTTTTAATTGTGCATCTTCCACAATGGCTGCAATTTCTTTTGGGTTAAGCCGCCAATTCAATGGTAAAAAAACCGCACCTGTTTTAAAACAAGCAAACAATAAATCTAATATTGCAATATCATTTGGCG  >  NC_007793/275005‑275149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑