Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,602,262	T→C	P69P (CCA→CCG)	SAUSA300_RS07910 ←	ADP‑ribose pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,602,262	0	T	C	100.0%	207.5 / NA	81	P69P (CCA→CCG)	SAUSA300_RS07910	ADP‑ribose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/80);  total (1/80)

CTTTGGCAATATATCCTGTTTCTTCTTCTAATTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAATGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCACCATTATGATAA  >  NC_007793/1602165‑1602352                                                                                                  |                                                                                           ctTTGGCAATATATCCTGTTTCTTCTTCTAATTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACgg                                                                                          <  1:266497/100‑1 (MQ=255)   ttGGCAATATATCCTGTTTCTTCTTCTAATTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGtt                                                                                        <  1:3581560/100‑1 (MQ=255)    tGGCAATATATCCTGTTTCTTCTTCTAATTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGttg                                                                                       <  1:52051/100‑2 (MQ=255)              tCCTGTTTCTTCTTCTAATTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGt                                                                             >  1:5858551/1‑100 (MQ=255)               ccTGTTTCTTCTTCTAATTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTgggg                                                                            <  1:2696081/100‑3 (MQ=255)                cTGTTTCTTCTTCTAATTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGttt                                                                           <  1:1711742/100‑1 (MQ=255)                 tGTTTCCTCTTCTAATTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTa                                                                          <  1:1847916/100‑1 (MQ=255)                         gatctAATTCCCGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTg                                                                  <  1:1037820/98‑1 (MQ=255)                         gatctAATTCACGTCTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTg                                                                  <  1:4818809/98‑1 (MQ=255)                          gatctaTTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTgc                                                                 <  1:2741675/95‑2 (MQ=255)                             tAATTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTc                                                              <  1:629279/100‑1 (MQ=255)                              aaTTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCa                                                             <  1:4121007/100‑1 (MQ=255)                                  taCGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTag                                                         <  1:3244641/99‑2 (MQ=255)                                     gTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCCGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATAc                                                      <  1:5475034/100‑1 (MQ=255)                                      ttttGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTCCCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACg                                                     <  1:910367/100‑1 (MQ=255)                                            cttccgatctctGTCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGActtca                                               <  1:5868759/92‑2 (MQ=255)                                             ttccgatctctATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGActtctt                                              <  1:1264364/93‑1 (MQ=255)                                                 tccgatcttctcCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCtttttt                                          <  1:5270622/92‑1 (MQ=255)                                                 gatctctATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCtttttt                                          <  1:4212260/97‑1 (MQ=255)                                                 cgatctcaTCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCtttttt                                          <  1:4129742/93‑1 (MQ=255)                                                 cGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCtttttt                                          <  1:2969959/100‑1 (MQ=255)                                                        tccTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTCTAGGTGTc                                   <  1:1304664/98‑1 (MQ=255)                                                        attttCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGgtgtg                                   <  1:3733499/97‑2 (MQ=255)                                                        aTCTTTATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTc                                   <  1:6455710/100‑1 (MQ=255)                                                        aTCTTCATCCTCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTc                                   <  1:2495575/100‑1 (MQ=255)                                                        aTCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTTCTTGTTTTCGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTc                                   <  1:4005892/100‑1 (MQ=255)                                                        aTCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTc                                   <  1:5184585/100‑1 (MQ=255)                                                        aTCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTc                                   <  1:4747968/100‑1 (MQ=255)                                                        aTCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTc                                   <  1:5379523/100‑1 (MQ=255)                                                        aTCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTc                                   <  1:543665/100‑1 (MQ=255)                                                        aTCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTc                                   <  1:2103504/100‑1 (MQ=255)                                                         tCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGgtgccc                                  <  1:4667603/100‑4 (MQ=255)                                                         tCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCCTTTTTAGGTGTcc                                  <  1:6104724/100‑2 (MQ=255)                                                          cTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCat                                 <  1:4489931/100‑2 (MQ=255)                                                          cTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCAc                                 <  1:1239118/100‑1 (MQ=255)                                                           ttCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACt                                <  1:885072/100‑1 (MQ=255)                                                             catcatCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGc                              <  1:4631377/100‑1 (MQ=255)                                                             catcatCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGc                              <  1:3976982/100‑1 (MQ=255)                                                             catcatCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGc                              <  1:3965368/100‑1 (MQ=255)                                                             catcatCTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGc                              <  1:1470505/100‑1 (MQ=255)                                                             caccatcTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGc                              <  1:2772585/97‑1 (MQ=255)                                                              atcatcTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGcc                             <  1:1443475/100‑2 (MQ=255)                                                               ttgtcttctAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATGCGACTTCTTTTTTAGGTGTCACTGcac                            <  1:3212908/97‑1 (MQ=255)                                                               ttatcTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGcac                            <  1:2662427/98‑1 (MQ=255)                                                               tcatcTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGcat                            <  1:5548313/100‑2 (MQ=255)                                                               tcatcTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGcac                            <  1:4581495/100‑1 (MQ=255)                                                               tcatcTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTCACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGcac                            <  1:2777136/100‑1 (MQ=255)                                                                catcTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGcacg                           <  1:5759059/100‑2 (MQ=255)                                                                catcTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGcaca                           <  1:4418590/100‑1 (MQ=255)                                                                catcTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGcaca                           <  1:2918583/100‑1 (MQ=255)                                                                 atcTTCTAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACaa                          <  1:6534799/100‑1 (MQ=255)                                                                  tcttctAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGcacagt                         <  1:6299899/100‑3 (MQ=255)                                                                  tcttctAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACaat                         <  1:2379758/100‑2 (MQ=255)                                                                  tcttctAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACaag                         <  1:5509341/100‑2 (MQ=255)                                                                   cttctAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAAc                        <  1:5943301/100‑1 (MQ=255)                                                                   cttctAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAAc                        <  1:5668922/100‑1 (MQ=255)                                                                   cttctAATTTACCAGCTGGAATTTCTAGCAACGGTTCTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAAc                        <  1:1334977/100‑1 (MQ=255)                                                                      ctAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGc                     <  1:1423061/100‑1 (MQ=255)                                                                       tgaTTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCa                    <  1:6189479/98‑1 (MQ=255)                                                                       tAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACTATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCa                    <  1:5580838/100‑1 (MQ=255)                                                                       tAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCa                    <  1:3804851/100‑1 (MQ=255)                                                                       tAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCa                    <  1:3501080/100‑1 (MQ=255)                                                                       tAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCa                    <  1:3218049/100‑1 (MQ=255)                                                                       tAATTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCa                    <  1:3112141/100‑1 (MQ=255)                                                                       tAACTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCa                    <  1:3015849/100‑1 (MQ=255)                                                                        aaTTTGCCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATTCTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCaa                   <  1:6129986/100‑1 (MQ=255)                                                                        aaTTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCaa                   <  1:2908838/100‑1 (MQ=255)                                                                        aaTTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGGTTACGATGCTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCaa                   <  1:83994/100‑1 (MQ=255)                                                                         aTTTACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAAc                  <  1:3158222/100‑1 (MQ=255)                                                                           ttACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTg                <  1:1486549/100‑1 (MQ=255)                                                                            tACCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGc               <  1:1594920/100‑1 (MQ=255)                                                                            tACCAGCTGGAATTTCTAGCAACGGTTTGTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGc               <  1:2956356/100‑1 (MQ=255)                                                                             aCCAGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCa              <  1:5602738/100‑1 (MQ=255)                                                                                aGCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCACCa           <  1:4212399/100‑1 (MQ=255)                                                                                 gCTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCagggc          <  1:1123004/100‑5 (MQ=255)                                                                                  cTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCACCAtt         <  1:5875469/100‑1 (MQ=255)                                                                                  cTGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCACCAtt         <  1:2342221/100‑1 (MQ=255)                                                                                   tGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCGCCATTa        <  1:3785143/100‑1 (MQ=255)                                                                                   tGGAATTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCACCAttg        <  1:2103652/100‑2 (MQ=255)                                                                                       aTTTCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCACCATTATGAt    <  1:2938434/100‑1 (MQ=255)                                                                                         ttCTAGCAACGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCACCATTATGATaa  <  1:4411448/100‑1 (MQ=255)                                                                                                  |                                                                                           CTTTGGCAATATATCCTGTTTCTTCTTCTAATTCACGTTTTGCCGCTTCGACTCTATCTTCATCATCTTCTAATTTACCAGCTGGAATTTCTAGCAATGGTTTTTCTACTGGTTTACGATACTGTTTCACTAATACGACTTCTTTTTTAGGTGTCACTGCACAAACAGCAACTGCACCATTATGATAA  >  NC_007793/1602165‑1602352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑