Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,590,674	A→C	intergenic (‑67/‑59)	SAUSA300_RS07850 ← / → SAUSA300_RS07855	hypothetical protein/integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,590,674	0	A	C	100.0%	15.2 / NA	8	intergenic (‑67/‑59)	SAUSA300_RS07850/SAUSA300_RS07855	hypothetical protein/integrase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/1);  total (7/1)

AGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACACATTTAGTACCTAGTACTAAATTACGGGTAGCCCGACTACCCTTATTATTTTTTAATATTTTAT  >  NC_007793/1590582‑1590702                                                                                             |                             agaagCGCCCCTAGAAATTGAGTCATCTTATCAACTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCt                       >  1:3836803/1‑100 (MQ=255)    cgcgcCCCTAGAAATTGAGTCATCTTACCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCttat                    >  1:6396978/2‑100 (MQ=255)      ggcCCCTAGAAATTGAGTCATCTTATCACCTACCTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCttatta                  >  1:1405445/2‑100 (MQ=255)      agcCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCttatta                  >  1:1532255/2‑100 (MQ=255)       gcCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCTtattat                 >  1:107435/1‑100 (MQ=255)       gcCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCTtattat                 >  1:3338856/1‑100 (MQ=255)                     aGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCTTATTAttttttgccaattct   >  1:3270340/1‑91 (MQ=25)                      gTCATCTTATCACCTACATTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCTTATTAttttttgccaattttg  <  1:4430410/100‑11 (MQ=21)                                                                                             |                             AGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACACATTTAGTACCTAGTACTAAATTACGGGTAGCCCGACTACCCTTATTATTTTTTAATATTTTAT  >  NC_007793/1590582‑1590702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑