Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,590,639	C→T	intergenic (‑32/‑94)	SAUSA300_RS07850 ← / → SAUSA300_RS07855	hypothetical protein/integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,590,639	0	C	T	100.0%	41.0 / NA	16	intergenic (‑32/‑94)	SAUSA300_RS07850/SAUSA300_RS07855	hypothetical protein/integrase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/2);  total (14/2)

AAACTCCTGTAAGAAGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACACATTTAGTACCTAGTACTAAATTACGGGTAGCCCGACTACCCTTATTATTTTTTAATATTTTAT  >  NC_007793/1590568‑1590702                                                                        |                                                                aaaCTCCTGTAAGAAGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGt                                     <  1:3017297/100‑1 (MQ=255)    gcccTGTAAGAAGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGc                                  >  1:1488010/3‑100 (MQ=255)      ccTGTAAGAAGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGccc                                >  1:2009370/1‑100 (MQ=255)      ccTGTAAGAAGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGccc                                >  1:2661552/1‑100 (MQ=255)      ccTGTAAGAAGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGccc                                >  1:6051966/1‑100 (MQ=255)      ccTGTAAGAAGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGccc                                >  1:79409/1‑100 (MQ=255)         gTAAGAAGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCgcc                             >  1:1585598/1‑98 (MQ=255)         cccagaagaagCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCgcc                             >  1:5599681/4‑98 (MQ=255)               agaagCGCCCCTAGAAATTGAGTCATCTTATCAACTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCt                       >  1:3836803/1‑100 (MQ=255)                  cgcgcCCCTAGAAATTGAGTCATCTTACCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCttat                    >  1:6396978/2‑100 (MQ=255)                    ggcCCCTAGAAATTGAGTCATCTTATCACCTACCTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCttatta                  >  1:1405445/2‑100 (MQ=255)                    agcCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCttatta                  >  1:1532255/2‑100 (MQ=255)                     gcCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCTtattat                 >  1:107435/1‑100 (MQ=255)                     gcCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCTtattat                 >  1:3338856/1‑100 (MQ=255)                                   aGTCATCTTATCACCTACTTTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCTTATTAttttttgccaattct   >  1:3270340/1‑91 (MQ=25)                                    gTCATCTTATCACCTACATTTTATTTTATTATAACATATTTAGTACCTAGTACTAAATTTTGGGTAGCCCGCCTACCCTTATTAttttttgccaattttg  <  1:4430410/100‑11 (MQ=21)                                                                        |                                                                AAACTCCTGTAAGAAGAAGCGCCCCTAGAAATTGAGTCATCTTATCACCTACTTTTTATTTTATTATAACACATTTAGTACCTAGTACTAAATTACGGGTAGCCCGACTACCCTTATTATTTTTTAATATTTTAT  >  NC_007793/1590568‑1590702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑