Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,590,508	C→T	V34I (GTT→ATT)	SAUSA300_RS07850 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,590,508	0	C	T	100.0%	150.4 / NA	56	V34I (GTT→ATT)	SAUSA300_RS07850	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/44);  total (12/44)

CAACATTATTAAAAATTATAAAACTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAACCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAGAAATTGAG  >  NC_007793/1590424‑1590603                                                                                     |                                                                                                 cagcATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCact                                                                                  >  1:4676678/4‑100 (MQ=255)      ttattaAAAATTATAAAAGTATAAAACCATAGCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTGCactaacta                                                                             <  1:1036969/100‑1 (MQ=255)           aaaaaTTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCt                                                                        >  1:6126126/1‑100 (MQ=255)             aaaTTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTAt                                                                      <  1:2769763/100‑1 (MQ=255)              aaTTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATc                                                                     <  1:3042666/100‑1 (MQ=255)               aTTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCa                                                                    <  1:1719489/100‑1 (MQ=255)                    aaaagtataaaacCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTgcc                                                               <  1:5914589/100‑3 (MQ=255)                    aaaagtataaaacCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGtt                                                               <  1:3825417/100‑1 (MQ=255)                    aaaagtataaaacCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGtt                                                               <  1:5827180/100‑1 (MQ=255)                    aaaagtataaaacCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGtt                                                               <  1:3097666/100‑1 (MQ=255)                    aaaagtataaaacCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGtt                                                               <  1:4271845/100‑1 (MQ=255)                                      cAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATACTCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATg                                             >  1:6309141/1‑100 (MQ=255)                                       caaCATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGt                                            >  1:1743731/2‑100 (MQ=255)                                       aaaCATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGt                                            <  1:1075422/100‑1 (MQ=255)                                       aaaCATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGt                                            <  1:6174333/100‑1 (MQ=255)                                        aaCATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTa                                           <  1:5373395/100‑1 (MQ=255)                                         tcATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTaa                                          >  1:2598666/2‑100 (MQ=255)                                         aCATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTaa                                          >  1:45415/1‑100 (MQ=255)                                                        aaaaaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCtaag                           <  1:6459513/100‑4 (MQ=255)                                                        aaaaaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTa                           <  1:4332067/100‑1 (MQ=255)                                                        aaaaaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTa                           <  1:3864543/100‑1 (MQ=255)                                                        aaaaaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTa                           <  1:4763082/100‑1 (MQ=255)                                                        aaaaaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTa                           <  1:618913/100‑1 (MQ=255)                                                        aaaaaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTa                           <  1:1876667/100‑1 (MQ=255)                                                        aaaaaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTa                           <  1:2022095/100‑1 (MQ=255)                                                        aaaaaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTa                           <  1:3642780/100‑1 (MQ=255)                                                          aaaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTaag                         >  1:6565521/1‑100 (MQ=255)                                                           aaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTaaga                        <  1:5562866/100‑1 (MQ=255)                                                           aaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTaaga                        <  1:1330316/100‑1 (MQ=255)                                                           aaTCAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTaaga                        <  1:1285418/100‑1 (MQ=255)                                                              cAATAATACAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTaagaaga                     <  1:4552976/100‑1 (MQ=255)                                                                     aCAGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTACGTAACCTAAAACGCCTGTaagaagaaggcaga              <  1:5460521/100‑6 (MQ=255)                                                                       aGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGcccca            <  1:2492434/100‑2 (MQ=255)                                                                       aGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCt            <  1:5435564/100‑1 (MQ=255)                                                                       aGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCt            <  1:5121485/100‑1 (MQ=255)                                                                       aGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCt            <  1:2168315/100‑1 (MQ=255)                                                                       aGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCt            <  1:6525725/100‑1 (MQ=255)                                                                       aGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCACCCCt            <  1:4027784/100‑1 (MQ=255)                                                                       aGGAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCACCCCt            <  1:1312840/100‑1 (MQ=255)                                                                         gAGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAg          <  1:3093173/100‑1 (MQ=255)                                                                          aGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCtgca         <  1:4544443/100‑4 (MQ=255)                                                                          aGTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAGa         <  1:4665061/100‑1 (MQ=255)                                                                           gTATTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAGag        <  1:246956/100‑2 (MQ=255)                                                                             aTTGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCtccgctg      <  1:4511048/100‑7 (MQ=255)                                                                               tGATAATCTTGTTTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGccccagatcgga     >  1:753677/1‑92 (MQ=255)                                                                               tGATAATCTTG‑TTTTTTTCACTAAGTAAGCCTAACATTGTGAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAGAAAGTg    <  1:1364097/100‑1 (MQ=255)                                                                               tGATAATCTTG‑TTTTTTTCACTAACTAAACCTCTCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGccccagatcggaa    >  1:3465329/1‑91 (MQ=255)                                                                               tGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCTTTGTTAGATATTTATATGGTATGTAACCTATTACTCCTGTAAGAAGAAGCACCCCTAGatcgga    >  1:2540417/1‑95 (MQ=255)                                                                               tGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAGGCGCCCCTAGAAATTg    <  1:3267402/100‑1 (MQ=255)                                                                               tGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGccccagatcggaa    >  1:3274221/1‑91 (MQ=255)                                                                               tGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAGAAATTg    <  1:5205719/100‑1 (MQ=255)                                                                               tGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAGAAATTg    <  1:4085229/100‑1 (MQ=255)                                                                               tGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAGAAATTg    <  1:6519296/100‑1 (MQ=255)                                                                               tGATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCACCAGatcgga    >  1:4420364/1‑95 (MQ=255)                                                                                gATAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAGAAATTGa   <  1:3994660/100‑1 (MQ=255)                                                                                 aTAATCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAGAAATTGAg  <  1:2910978/100‑1 (MQ=255)                                                                                     |                                                                                                 CAACATTATTAAAAATTATAAAACTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAACCTTG‑TTTTTTTCACTAACTAAACCTATCATTGTTAGATATTTATATGGTATGTAACCTAAAACTCCTGTAAGAAGAAGCGCCCCTAGAAATTGAG  >  NC_007793/1590424‑1590603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑