Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,590,447	C→G	S54T (AGT→ACT)	SAUSA300_RS07850 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,590,447	0	C	G	100.0%	147.5 / NA	54	S54T (AGT→ACT)	SAUSA300_RS07850	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (34/20);  total (34/20)

AAATAGAAACAAAATATTTGCTTTTAGACCTGTAAGCAACTGAATTAAATTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAACTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAACCTTGTTTTTTTCACTAACTAAACCTATCATTGTT  >  NC_007793/1590359‑1590542                                                                                         |                                                                                                aattaGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCat                                                                                      <  1:5204338/97‑1 (MQ=255)    taGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATc                                                                                   <  1:2684838/100‑1 (MQ=255)        aaCCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAAc                                                                               >  1:2339413/1‑100 (MQ=255)         accaaaTATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACa                                                                              >  1:4058947/4‑100 (MQ=255)                       tttAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaa                                                                >  1:4574330/1‑100 (MQ=255)                       tctAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaa                                                                >  1:5330861/3‑100 (MQ=255)                        ttAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaa                                                               >  1:5553951/1‑100 (MQ=255)                        ctAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaa                                                               >  1:4950522/2‑100 (MQ=255)                         tAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaaa                                                              >  1:3738604/1‑100 (MQ=255)                          aGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaaaa                                                             >  1:1589454/1‑100 (MQ=255)                          aGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaaaa                                                             >  1:6421865/1‑100 (MQ=255)                          aGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaaaa                                                             >  1:6414946/1‑100 (MQ=255)                          aGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaaaa                                                             >  1:2842433/1‑100 (MQ=255)                           ctccTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAAt                                                            >  1:4250992/3‑100 (MQ=255)                             tcTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCa                                                          >  1:1945867/2‑100 (MQ=255)                             ccTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCa                                                          >  1:1480974/1‑100 (MQ=255)                               agTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaat                                                        >  1:408398/2‑100 (MQ=255)                                gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAACGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata                                                       >  1:823049/1‑100 (MQ=255)                                gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata                                                       >  1:2676545/1‑100 (MQ=255)                                gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata                                                       >  1:1674377/1‑100 (MQ=255)                                gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata                                                       >  1:2085249/1‑100 (MQ=255)                                gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata                                                       >  1:4286162/1‑100 (MQ=255)                                gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGCATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata                                                       >  1:2022988/1‑100 (MQ=255)                                  taGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataac                                                     >  1:2716370/2‑99 (MQ=255)                                  aaGCAACTGTAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat                                                     >  1:4109064/1‑100 (MQ=255)                                  aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAaaaaaagatcggaag                                                     >  1:5908352/1‑91 (MQ=255)                                  aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat                                                     >  1:6619211/1‑100 (MQ=255)                                  aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat                                                     >  1:5952962/1‑100 (MQ=255)                                  aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat                                                     >  1:2214922/1‑100 (MQ=255)                                  aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat                                                     >  1:5563286/1‑100 (MQ=255)                                  aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat                                                     >  1:1783482/1‑100 (MQ=255)                                   aGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGGAAAAATCAataata                                                    >  1:4865290/1‑100 (MQ=255)                                    gCAACTGAAGTAAATTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATAc                                                   <  1:4827636/100‑1 (MQ=255)                                    gCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATAc                                                   >  1:4152261/1‑100 (MQ=255)                                    gCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGCTTCAATAGAAAAAATCAATAATAc                                                   <  1:1856120/100‑1 (MQ=255)                                       aCTGAAGTAAACTCAAATTTTTTAAATCACCATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg                                                <  1:4533224/100‑1 (MQ=255)                                       aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg                                                <  1:2451610/100‑1 (MQ=255)                                       aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg                                                <  1:4872154/100‑1 (MQ=255)                                       aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg                                                <  1:5538684/100‑1 (MQ=255)                                       aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg                                                <  1:5983330/100‑1 (MQ=255)                                       aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg                                                <  1:6423153/100‑1 (MQ=255)                                       aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg                                                <  1:2739568/100‑1 (MQ=255)                                       aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg                                                <  1:3468449/100‑1 (MQ=255)                                       aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg                                                <  1:3815335/100‑1 (MQ=255)                                       aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg                                                <  1:4194137/100‑1 (MQ=255)                                          gAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGt                                             <  1:2411416/100‑1 (MQ=255)                                          gAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGt                                             <  1:2287657/100‑1 (MQ=255)                                               aaaCTCAAACTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGa                                        >  1:1998058/1‑100 (MQ=255)                                                                  cagcATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTTCact                     >  1:4676678/4‑100 (MQ=255)                                                                       ttattaAAAATTATAAAAGTATAAAACCATAGCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTGCactaacta                <  1:1036969/100‑1 (MQ=255)                                                                            aaaaaTTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTTCACTAACTAAACCt           >  1:6126126/1‑100 (MQ=255)                                                                              aaaTTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTTCACTAACTAAACCTAt         <  1:2769763/100‑1 (MQ=255)                                                                               aaTTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTTCACTAACTAAACCTATc        <  1:3042666/100‑1 (MQ=255)                                                                                aTTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTTCACTAACTAAACCTATCa       <  1:1719489/100‑1 (MQ=255)                                                                                     aaaagtataaaacCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTTCACTAACTAAACCTATCATTgcc  <  1:5914589/100‑3 (MQ=255)                                                                                     aaaagtataaaacCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTTCACTAACTAAACCTATCATTGtt  <  1:5827180/100‑1 (MQ=255)                                                                                     aaaagtataaaacCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTTCACTAACTAAACCTATCATTGtt  <  1:4271845/100‑1 (MQ=255)                                                                                     aaaagtataaaacCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTTCACTAACTAAACCTATCATTGtt  <  1:3097666/100‑1 (MQ=255)                                                                                     aaaagtataaaacCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAATCTTGTTTTTTTCACTAACTAAACCTATCATTGtt  <  1:3825417/100‑1 (MQ=255)                                                                                         |                                                                                                AAATAGAAACAAAATATTTGCTTTTAGACCTGTAAGCAACTGAATTAAATTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAACTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGTATTGATAACCTTGTTTTTTTCACTAACTAAACCTATCATTGTT  >  NC_007793/1590359‑1590542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑