Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,590,403	T→G	I69L (ATT→CTT)	SAUSA300_RS07850 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,590,403	0	T	G	100.0%	143.6 / NA	54	I69L (ATT→CTT)	SAUSA300_RS07850	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (38/16);  total (38/16)

TAAATACAAACACTGTTAAAACAAAAATAAATAGAAACAAAATATTTGCTTTTAGACCTGTAAGCAACTGAATTAAATTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAACTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGT  >  NC_007793/1590331‑1590499                                                                         |                                                                                                 tAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACatt                                                                       >  1:1796445/1‑100 (MQ=255)      aCAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaa                                                                  >  1:4737058/1‑100 (MQ=255)      aCAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaa                                                                  >  1:1006507/1‑100 (MQ=255)        aagcacTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaaaa                                                                >  1:2611818/4‑100 (MQ=255)        aagcacTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaaaa                                                                >  1:228781/4‑100 (MQ=255)          gcacTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAAt                                                              >  1:5147093/2‑100 (MQ=255)               gTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATtataa                                                         >  1:542338/1‑100 (MQ=255)                             aattaGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCat                                           <  1:5204338/97‑1 (MQ=255)                                taGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATc                                        <  1:2684838/100‑1 (MQ=255)                                    aaCCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAAc                                    >  1:2339413/1‑100 (MQ=255)                                     accaaaTATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACa                                   >  1:4058947/4‑100 (MQ=255)                                                   tttAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaa                     >  1:4574330/1‑100 (MQ=255)                                                   tctAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaa                     >  1:5330861/3‑100 (MQ=255)                                                    ttAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaa                    >  1:5553951/1‑100 (MQ=255)                                                    ctAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaa                    >  1:4950522/2‑100 (MQ=255)                                                     tAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaaa                   >  1:3738604/1‑100 (MQ=255)                                                      aGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaaaa                  >  1:6414946/1‑100 (MQ=255)                                                      aGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaaaa                  >  1:6421865/1‑100 (MQ=255)                                                      aGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaaaa                  >  1:1589454/1‑100 (MQ=255)                                                      aGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGaaaaaa                  >  1:2842433/1‑100 (MQ=255)                                                       ctccTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAAt                 >  1:4250992/3‑100 (MQ=255)                                                         tcTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCa               >  1:1945867/2‑100 (MQ=255)                                                         ccTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCa               >  1:1480974/1‑100 (MQ=255)                                                           agTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaat             >  1:408398/2‑100 (MQ=255)                                                            gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAACGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata            >  1:823049/1‑100 (MQ=255)                                                            gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata            >  1:1674377/1‑100 (MQ=255)                                                            gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata            >  1:2085249/1‑100 (MQ=255)                                                            gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata            >  1:4286162/1‑100 (MQ=255)                                                            gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata            >  1:2676545/1‑100 (MQ=255)                                                            gTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGCATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaata            >  1:2022988/1‑100 (MQ=255)                                                              taGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataac          >  1:2716370/2‑99 (MQ=255)                                                              aaGCAACTGTAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat          >  1:4109064/1‑100 (MQ=255)                                                              aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAaaaaaagatcggaag          >  1:5908352/1‑91 (MQ=255)                                                              aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat          >  1:5563286/1‑100 (MQ=255)                                                              aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat          >  1:2214922/1‑100 (MQ=255)                                                              aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat          >  1:5952962/1‑100 (MQ=255)                                                              aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat          >  1:1783482/1‑100 (MQ=255)                                                              aaGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCaataat          >  1:6619211/1‑100 (MQ=255)                                                               aGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGGAAAAATCAataata         >  1:4865290/1‑100 (MQ=255)                                                                gCAACTGAAGTAAATTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATAc        <  1:4827636/100‑1 (MQ=255)                                                                gCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATAc        >  1:4152261/1‑100 (MQ=255)                                                                gCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGCTTCAATAGAAAAAATCAATAATAc        <  1:1856120/100‑1 (MQ=255)                                                                   aCTGAAGTAAACTCAAATTTTTTAAATCACCATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg     <  1:4533224/100‑1 (MQ=255)                                                                   aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg     <  1:5983330/100‑1 (MQ=255)                                                                   aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg     <  1:6423153/100‑1 (MQ=255)                                                                   aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg     <  1:3468449/100‑1 (MQ=255)                                                                   aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg     <  1:5538684/100‑1 (MQ=255)                                                                   aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg     <  1:2451610/100‑1 (MQ=255)                                                                   aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg     <  1:4872154/100‑1 (MQ=255)                                                                   aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg     <  1:2739568/100‑1 (MQ=255)                                                                   aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg     <  1:4194137/100‑1 (MQ=255)                                                                   aCTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAgg     <  1:3815335/100‑1 (MQ=255)                                                                      gAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGt  <  1:2287657/100‑1 (MQ=255)                                                                      gAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGt  <  1:2411416/100‑1 (MQ=255)                                                                         |                                                                                                 TAAATACAAACACTGTTAAAACAAAAATAAATAGAAACAAAATATTTGCTTTTAGACCTGTAAGCAACTGAATTAAATTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAACTATAAAACCATATCAAACATGTTTCAATAGAAAAAATCAATAATACAGGAGT  >  NC_007793/1590331‑1590499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑