Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,590,369	A→C	L80W (TTG→TGG)	SAUSA300_RS07850 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,590,369	0	A	C	100.0%	54.7 / NA	22	L80W (TTG→TGG)	SAUSA300_RS07850	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/10);  total (12/10)

AAACTTTCTGGTTATATTAATTAACCAGATAATAAATTTAACAATTAAAGGATTAAATACAAACACTGTTAAAACAAAAATAAATAGAAACAAAATATTTGCTTTTAGACCTGTAAGCAACTGAATTAAATTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAACTATAAAACCATATCAAAC  >  NC_007793/1590278‑1590465                                                                                            |                                                                                                 aaaCTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATAttt                                                                                          >  1:753239/1‑100 (MQ=255)       tcttgtttcattaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCtttt                                                                                    <  1:5105186/91‑1 (MQ=255)                attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                                                           <  1:6058264/100‑1 (MQ=255)                attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                                                           <  1:4150845/100‑1 (MQ=255)                attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                                                           <  1:4260246/100‑1 (MQ=255)                attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                                                           <  1:4519428/100‑1 (MQ=255)                 ttaattaaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTaa                                                                          <  1:5713920/100‑1 (MQ=255)                   aattaaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGc                                                                        <  1:2978896/100‑1 (MQ=255)                   aattaaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTGAAAACAAAAATAATTAGAACCCAAATATTTGCTTTTAGACCTGCAAGc                                                                        <  1:6075693/100‑1 (MQ=255)                     ataaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCaa                                                                      >  1:3887011/2‑100 (MQ=255)                       aaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACt                                                                    >  1:550760/1‑100 (MQ=255)                         ccAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGa                                                                  >  1:5252323/1‑100 (MQ=255)                                                      tAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACatt                                     >  1:1796445/1‑100 (MQ=255)                                                           aCAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaa                                >  1:4737058/1‑100 (MQ=255)                                                           aCAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaa                                >  1:1006507/1‑100 (MQ=255)                                                             aagcacTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaaaa                              >  1:2611818/4‑100 (MQ=255)                                                             aagcacTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaaaa                              >  1:228781/4‑100 (MQ=255)                                                               gcacTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAAt                            >  1:5147093/2‑100 (MQ=255)                                                                    gTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATtataa                       >  1:542338/1‑100 (MQ=255)                                                                                  aattaGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCat         <  1:5204338/97‑1 (MQ=255)                                                                                     taGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATc      <  1:2684838/100‑1 (MQ=255)                                                                                         aaCCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAAGTATAAAACCATATCAAAc  >  1:2339413/1‑100 (MQ=255)                                                                                            |                                                                                                 AAACTTTCTGGTTATATTAATTAACCAGATAATAAATTTAACAATTAAAGGATTAAATACAAACACTGTTAAAACAAAAATAAATAGAAACAAAATATTTGCTTTTAGACCTGTAAGCAACTGAATTAAATTCAAATTTTTTAAATCAACATTATTAAAAATTATAAAACTATAAAACCATATCAAAC  >  NC_007793/1590278‑1590465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑