Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,590,361	A→T	F83I (TTT→ATT)	SAUSA300_RS07850 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,590,361	0	A	T	100.0%	59.2 / NA	22	F83I (TTT→ATT)	SAUSA300_RS07850	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/11);  total (11/11)

CCAATTTCATAAACTTTCTGGTTATATTAATTAACCAGATAATAAATTTAACAATTAAAGGATTAAATACAAACACTGTTAAAACAAAAATAAATAGAAACAAAATATTTGCTTTTAGACCTGTAAGCAACTGAATTAAATTCAAATTTTTTAAATCAACATTATTAAAAATTATAA  >  NC_007793/1590268‑1590444                                                                                              |                                                                                    ccAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGagg                                                                               <  1:6264852/100‑3 (MQ=255)    aattCATAAACTTTTTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAAcca                                                                            <  1:4927965/98‑3 (MQ=255)    aattCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAAcca                                                                            <  1:2649252/98‑3 (MQ=255)           aaaCTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATAttt                                                                     >  1:753239/1‑100 (MQ=255)                 tcttgtttcattaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCtttt                                                               <  1:5105186/91‑1 (MQ=255)                          attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                                      <  1:4150845/100‑1 (MQ=255)                          attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                                      <  1:4260246/100‑1 (MQ=255)                          attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                                      <  1:4519428/100‑1 (MQ=255)                          attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                                      <  1:6058264/100‑1 (MQ=255)                           ttaattaaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTaa                                                     <  1:5713920/100‑1 (MQ=255)                             aattaaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGc                                                   <  1:2978896/100‑1 (MQ=255)                             aattaaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTGAAAACAAAAATAATTAGAACCCAAATATTTGCTTTTAGACCTGCAAGc                                                   <  1:6075693/100‑1 (MQ=255)                               ataaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCaa                                                 >  1:3887011/2‑100 (MQ=255)                                 aaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACt                                               >  1:550760/1‑100 (MQ=255)                                   ccAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGa                                             >  1:5252323/1‑100 (MQ=255)                                                                tAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACatt                >  1:1796445/1‑100 (MQ=255)                                                                     aCAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaa           >  1:1006507/1‑100 (MQ=255)                                                                     aCAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaa           >  1:4737058/1‑100 (MQ=255)                                                                       aagcacTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaaaa         >  1:228781/4‑100 (MQ=255)                                                                       aagcacTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaaaa         >  1:2611818/4‑100 (MQ=255)                                                                         gcacTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAAt       >  1:5147093/2‑100 (MQ=255)                                                                              gTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTAAAAATtataa  >  1:542338/1‑100 (MQ=255)                                                                                              |                                                                                    CCAATTTCATAAACTTTCTGGTTATATTAATTAACCAGATAATAAATTTAACAATTAAAGGATTAAATACAAACACTGTTAAAACAAAAATAAATAGAAACAAAATATTTGCTTTTAGACCTGTAAGCAACTGAATTAAATTCAAATTTTTTAAATCAACATTATTAAAAATTATAA  >  NC_007793/1590268‑1590444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑