Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,590,340	A→G	F90L (TTT→CTT)	SAUSA300_RS07850 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,590,340	0	A	G	100.0%	57.5 / NA	23	F90L (TTT→CTT)	SAUSA300_RS07850	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/14);  total (9/14)

CTTTTGTCTAATAAGCTTATACAATCCAATTTCATAAACTTTCTGGTTATATTAATTAACCAGATAATAAATTTAACAATTAAAGGATTAAATACAAACACTGTTAAAACAAAAATAAATAGAAACAAAATATTTGCTTTTAGACCTGTAAGCAACTGAATTAAATTCAAATTTTTTAAATCAACATTATTAA  >  NC_007793/1590243‑1590435                                                                                                  |                                                                                                ctTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGGATTTAACAATTAAAGGATTAAATACAAgca                                                                                               >  1:5106136/1‑100 (MQ=255)       tCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTa                                                                                         <  1:5150138/100‑1 (MQ=255)       tCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTa                                                                                         <  1:2840696/100‑1 (MQ=255)         taataaGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTaaa                                                                                       <  1:2341228/100‑1 (MQ=255)          aataaGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTaaaa                                                                                      >  1:7581/1‑100 (MQ=255)                   ataCAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAaaataa                                                                             >  1:1553138/1‑100 (MQ=255)                          ccAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGagg                                                                      <  1:6264852/100‑3 (MQ=255)                             aattCATAAACTTTTTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAAcca                                                                   <  1:4927965/98‑3 (MQ=255)                             aattCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAAcca                                                                   <  1:2649252/98‑3 (MQ=255)                                    aaaCTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATAttt                                                            >  1:753239/1‑100 (MQ=255)                                          tcttgtttcattaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCtttt                                                      <  1:5105186/91‑1 (MQ=255)                                                   attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                             <  1:4150845/100‑1 (MQ=255)                                                   attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                             <  1:4260246/100‑1 (MQ=255)                                                   attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                             <  1:4519428/100‑1 (MQ=255)                                                   attaattaACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTa                                             <  1:6058264/100‑1 (MQ=255)                                                    ttaattaaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTaa                                            <  1:5713920/100‑1 (MQ=255)                                                      aattaaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGc                                          <  1:2978896/100‑1 (MQ=255)                                                      aattaaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTGAAAACAAAAATAATTAGAACCCAAATATTTGCTTTTAGACCTGCAAGc                                          <  1:6075693/100‑1 (MQ=255)                                                        ataaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCaa                                        >  1:3887011/2‑100 (MQ=255)                                                          aaCCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACt                                      >  1:550760/1‑100 (MQ=255)                                                            ccAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGa                                    >  1:5252323/1‑100 (MQ=255)                                                                                         tAAATACAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACatt       >  1:1796445/1‑100 (MQ=255)                                                                                              aCAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaa  >  1:4737058/1‑100 (MQ=255)                                                                                              aCAAGCACTGTTAAAACAAAAATAATTAGAAACCAAATATTTGCTTTTAGACCTGTAAGCAACTGAAGTAAACTCAAATTTTTTAAATCAACATTATTaa  >  1:1006507/1‑100 (MQ=255)                                                                                                  |                                                                                                CTTTTGTCTAATAAGCTTATACAATCCAATTTCATAAACTTTCTGGTTATATTAATTAACCAGATAATAAATTTAACAATTAAAGGATTAAATACAAACACTGTTAAAACAAAAATAAATAGAAACAAAATATTTGCTTTTAGACCTGTAAGCAACTGAATTAAATTCAAATTTTTTAAATCAACATTATTAA  >  NC_007793/1590243‑1590435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑