Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,590,272	T→A	K112N (AAA→AAT)	SAUSA300_RS07850 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,590,272	0	T	A	100.0%	88.5 / NA	32	K112N (AAA→AAT)	SAUSA300_RS07850	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/23);  total (9/23)

GATTCTGTTTTCAAAGTCTTTTATAACTATAAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAATTTCATAAACTTTCTGGTTATATTAATTAACCAGATAATAAATTTAACAATTAAAGGATTAAATACAAACACTGTTAAAACAAAAATAAATAGAAA  >  NC_007793/1590176‑1590367                                                                                                 |                                                                                                gATTCTGTTTTCAAAGTCTTTAATAACTATAAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTc                                                                                              >  1:6477392/1‑100 (MQ=255)   ttCTGTTTTCAAAGTCTTTAATAACTATAAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCAt                                                                                            <  1:1578514/100‑1 (MQ=255)   ttCTGTTTTCAAAGTCTTTAATAACTATAAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCAt                                                                                            <  1:459804/100‑1 (MQ=255)       gTTTTCAAAGTCTTTAATAACTATAAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAAc                                                                                        <  1:1311993/100‑1 (MQ=255)             aaaGTCTTTAATAACTATAAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCtt                                                                                  <  1:2803566/100‑2 (MQ=255)                  cTTTAATAACTATAAATACTGGTTTACCGTTGTTACTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGtttc                                                                             <  1:4373173/100‑3 (MQ=255)                    ttaataACTATAAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGtttcat                                                                           >  1:760786/4‑96 (MQ=255)                             ataAATACTGGTTTACCGTTGTTATTAAACAGCTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAAcc                                                                  <  1:2052495/100‑1 (MQ=255)                             ataAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTGTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTGTCTTGTTTCATTAATTAAcc                                                                  <  1:4381741/100‑1 (MQ=255)                             ataAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAAcc                                                                  <  1:4371282/100‑1 (MQ=255)                             ataAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAAcc                                                                  <  1:2346551/100‑1 (MQ=255)                             ataAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAAcc                                                                  <  1:5418031/100‑1 (MQ=255)                             ataAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAAcc                                                                  <  1:2212743/100‑1 (MQ=255)                             ataAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAAcc                                                                  <  1:1932330/100‑1 (MQ=255)                             ataAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAAcc                                                                  <  1:1218352/100‑1 (MQ=255)                               aaaTACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAg                                                                >  1:5220307/1‑100 (MQ=255)                               aaaTACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTCGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAg                                                                <  1:1973361/100‑1 (MQ=255)                                aaTACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAgc                                                               <  1:1048710/100‑2 (MQ=255)                                 aTACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGat                                                              <  1:1118515/100‑1 (MQ=255)                                 aTACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGat                                                              <  1:2344385/100‑1 (MQ=255)                                      ggTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGataatga                                                         >  1:6271959/1‑98 (MQ=255)                                      ggTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGataatga                                                         >  1:2045981/1‑98 (MQ=255)                                                         acaacTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGAtt                                      <  1:3670082/100‑1 (MQ=255)                                                            acTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTaaa                                   >  1:4597020/1‑100 (MQ=255)                                                                   tctTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACaagc                            <  1:2585690/100‑3 (MQ=255)                                                                    ctTTTGTCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGGATTTAACAATTAAAGGATTAAATACAAgca                           >  1:5106136/1‑100 (MQ=255)                                                                          tCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTa                     <  1:2840696/100‑1 (MQ=255)                                                                          tCTAATAAGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTa                     <  1:5150138/100‑1 (MQ=255)                                                                            taataaGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTaaa                   <  1:2341228/100‑1 (MQ=255)                                                                             aataaGCTTATACAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTaaaa                  >  1:7581/1‑100 (MQ=255)                                                                                      ataCAATCCAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAaaataa         >  1:1553138/1‑100 (MQ=255)                                                                                             ccAAATTCATAAACTTTCTTGTTTCATTAATTAACCAGATAATGAATTTAACAATTAAAGGATTAAATACAAGCACTGTTAAAACAAAAATAATTAGagg  <  1:6264852/100‑3 (MQ=255)                                                                                                 |                                                                                                GATTCTGTTTTCAAAGTCTTTTATAACTATAAATACTGGTTTACCGTTGTTATTAAACAACTTGTCTCTTTTGTCTAATAAGCTTATACAATCCAATTTCATAAACTTTCTGGTTATATTAATTAACCAGATAATAAATTTAACAATTAAAGGATTAAATACAAACACTGTTAAAACAAAAATAAATAGAAA  >  NC_007793/1590176‑1590367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑