Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,589,954	A→C	K28N (AAA→AAC)	SAUSA300_RS07845 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,589,954	0	A	C	100.0%	124.1 / NA	47	K28N (AAA→AAC)	SAUSA300_RS07845	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/16);  total (31/16)

GTGGTGAGAATTTTATGGTAGATAAAAACAAAAAACAAGAAACTACTCGTAGTAACCCATTAAACAAAAGTTTTGAAAAGTCAGGCGCCAGCGAAAAATTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAGTATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGTCTACA  >  NC_007793/1589857‑1590047                                                                                                  |                                                                                              gtggtgAGAATTTTATGGCGGATAAAAACAAAAAACAAGAAGCTACTCGTAGTAAACCAATAAACAAAAGCTTTGAAAAGCCGGGTGCCAGCGAAAACtt                                                                                             >  1:2350372/1‑100 (MQ=255) gtggtgAGAATTTTATGGCGGATAAAAACAAAAAACAAGAAGATACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGAGCCAGCGAAAACtt                                                                                             >  1:4786197/1‑100 (MQ=255)   ggtgAGAATTTTATGGCGGATAAAAACAAAAAACAAGAAGCTACTCGTAGAAACCCAATAAACAAAAGTTTTGAAAAGCCGGGGGCCAGCGAAAACTTaa                                                                                           >  1:5107228/1‑100 (MQ=255)   ggtgAGAATTTTATGGCGGATAAAAACAAAAAACAAGAACCTCCTCGTTGTAACCCCATTAACAAAAGTTTTGAAAAGCCGGGAGCCAGCGAAAACTTaa                                                                                           >  1:3111504/1‑100 (MQ=255)             ttATGGCGGATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGGGCCAGCGAAAACTTAAAAAGCACttt                                                                                 >  1:3428327/1‑100 (MQ=255)             ctATGGCGGATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACttt                                                                                 >  1:3021768/2‑100 (MQ=255)              tgtggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTa                                                                                >  1:1009260/8‑100 (MQ=255)              tATGGCGGATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTa                                                                                >  1:4147905/1‑100 (MQ=255)              tATGGCGGATAAAAACAAAAAACAAGAAGCTACTCGCAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTa                                                                                >  1:4847659/1‑100 (MQ=255)               gtggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTAt                                                                               >  1:1486363/7‑100 (MQ=255)               ctggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTAt                                                                               >  1:4542906/7‑100 (MQ=255)               ctggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTAt                                                                               >  1:3590381/7‑100 (MQ=255)               atggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTAt                                                                               >  1:5030204/7‑100 (MQ=255)               atggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTAt                                                                               >  1:6167256/7‑100 (MQ=255)               atggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTAt                                                                               >  1:847219/7‑100 (MQ=255)                tggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATc                                                                              >  1:6307574/6‑100 (MQ=255)                tggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATc                                                                              <  1:2546694/95‑1 (MQ=255)                tggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGATTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATc                                                                              >  1:5653315/6‑100 (MQ=255)                tGGCAGATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATc                                                                              <  1:1395034/100‑1 (MQ=255)                 ggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCa                                                                             >  1:227284/5‑100 (MQ=255)                 ggcggATAAAAACAAAAAACAAGAAGCTACTCGTAGTAACCCAATAAACAAAAGCTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCa                                                                             >  1:141162/5‑100 (MQ=255)                           aacaaaaaacaaGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTa                                                                   <  1:6159713/100‑1 (MQ=255)                           aacaaaaaacaaGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTCTATCAGAAAAAGCTa                                                                   <  1:4160601/100‑1 (MQ=255)                                aaaacaaGAAGCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCAGAAAGAGCTAAGatc                                                              >  1:5303032/1‑98 (MQ=255)                                     aagaagCTACTCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGa                                                         >  1:365721/1‑100 (MQ=255)                                        tccgatctCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTa                                                      <  1:1631534/94‑1 (MQ=255)                                          gctactCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTaat                                                    >  1:2743385/2‑98 (MQ=255)                                          agtactCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTaat                                                    >  1:5917729/3‑98 (MQ=255)                                             actCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATatt                                                 >  1:2700871/1‑100 (MQ=255)                                              ctCGTAGTAACCCAATAAACAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATattc                                                <  1:1716201/100‑1 (MQ=255)                                                              aaaCAAAAGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATaaa                                >  1:3159360/1‑100 (MQ=255)                                                                    aaGTTTTGAAAAGCCGGGTGCCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCaag                          <  1:1511918/100‑2 (MQ=255)                                                                             gtgtgcgggtgcCAGCGAAAACTTAAAAAGCACTTTATCAGAAATAGCTAAGCAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATttgt                 >  1:4814450/11‑100 (MQ=255)                                                                                 ccgggtgcCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTgttgtt             <  1:2796794/94‑1 (MQ=255)                                                                                 ccgggtgcCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTgttgtt             >  1:4454824/7‑100 (MQ=255)                                                                                  cgggtgcCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGttt            <  1:2144643/95‑1 (MQ=255)                                                                                   gggtgtcAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTa           <  1:1604523/94‑1 (MQ=255)                                                                                   gggtgcCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTa           <  1:5193890/96‑1 (MQ=255)                                                                                       gcCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGTTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGt       >  1:576476/1‑100 (MQ=255)                                                                                       gcCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGt       <  1:4842213/100‑1 (MQ=255)                                                                                       gcCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGt       >  1:4050606/1‑100 (MQ=255)                                                                                       gcCAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGt       >  1:4010836/1‑100 (MQ=255)                                                                                        ccAGCGAAAACTTAAAAAGCACTTTAGCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGTc      >  1:6050457/1‑100 (MQ=255)                                                                                         cAGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGTCt     <  1:566346/100‑1 (MQ=255)                                                                                          aGCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGTCTa    <  1:3789678/100‑1 (MQ=255)                                                                                           gCGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGTCTAc   <  1:2217506/100‑1 (MQ=255)                                                                                            cGAAAACTTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAATATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGTCtgaa  <  1:1576743/100‑4 (MQ=255)                                                                                                  |                                                                                              GTGGTGAGAATTTTATGGTAGATAAAAACAAAAAACAAGAAACTACTCGTAGTAACCCATTAAACAAAAGTTTTGAAAAGTCAGGCGCCAGCGAAAAATTAAAAAGCACTTTATCAGAAAAAGCTAAGAAAAAAGATTAGTATTCATTCATTAAATATAAATCCAATTTAATTTGTTGTTTAAGGTCTACA  >  NC_007793/1589857‑1590047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑