Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	269,528	A→G	R164R (CGT→CGC)	SAUSA300_RS01195 ←	acetyl‑CoA acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	269,528	0	A	G	100.0%	60.0 / NA	25	R164R (CGT→CGC)	SAUSA300_RS01195	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/21);  total (4/21)

CTTGTATTGGAATAATTTCATCTTTGAACCGACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCACGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTAAGGTTGGATTGTTTGTG  >  NC_007793/269434‑269624                                                                                               |                                                                                                 cTTGTATTGGAATAATTTCATCTTTGAACCGACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTgc                                                                                             <  1:1857331/100‑2 (MQ=255)   cgTATTGGAATAATTTCATCTTTGAACCGACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTgggt                                                                                           <  1:5742419/99‑4 (MQ=255)               atTTCATCTTTGAACCGACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTggg                                                                               <  1:264857/100‑1 (MQ=255)               atTTCATCTTTGAACCGACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTggg                                                                               <  1:3938405/100‑1 (MQ=255)                 ttCATCTTTGAACCGACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGAt                                                                             <  1:1349523/100‑1 (MQ=255)                    aTCTTTGAACCGACTATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCt                                                                          <  1:4968939/100‑1 (MQ=255)                       tttGAACCGTCCATCACGTTGTGCGGCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACa                                                                       <  1:4447285/100‑1 (MQ=255)                        ctGAACCGACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACAt                                                                      >  1:939137/2‑100 (MQ=255)                                  taTCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCTAATTGGGATGCTACATTTTCAGCAGt                                                            <  1:4474042/99‑1 (MQ=255)                                    tCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCGTCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTa                                                          <  1:1309598/100‑1 (MQ=255)                                              cGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAgg                                                <  1:3448900/100‑1 (MQ=255)                                                tCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGat                                              <  1:1271401/100‑1 (MQ=255)                                                 cATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGata                                             <  1:6280651/100‑1 (MQ=255)                                                  aTAGGCACGTTGATGACTTCTGACAGCATAAGAATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGatat                                            >  1:1219940/1‑100 (MQ=255)                                                                 aCTCCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATatca                             >  1:6349755/1‑100 (MQ=255)                                                                       gACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCatat                       >  1:882581/1‑100 (MQ=255)                                                                          aGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGt                    <  1:4089482/100‑1 (MQ=255)                                                                          aGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGt                    <  1:849168/100‑1 (MQ=255)                                                                           gCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTa                   <  1:4005498/100‑1 (MQ=255)                                                                           gCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTa                   <  1:354123/100‑1 (MQ=255)                                                                           gCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTa                   <  1:4965467/100‑1 (MQ=255)                                                                           gCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTa                   <  1:5503370/100‑1 (MQ=255)                                                                            cATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTaa                  <  1:2094955/100‑1 (MQ=255)                                                                              tAAGCATCTGGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTAAgg                <  1:1724036/100‑1 (MQ=255)                                                                                            ttCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTAAGGTTGGATTGTTtgtg  <  1:524136/100‑1 (MQ=255)                                                                                               |                                                                                                 CTTGTATTGGAATAATTTCATCTTTGAACCGACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCACGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTAAGGTTGGATTGTTTGTG  >  NC_007793/269434‑269624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑