Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,586,582	T→A	pseudogene (90/162 nt)	SAUSA300_RS07800 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,586,582	0	T	A	100.0%	10.0 / NA	6	pseudogene (90/162 nt)	SAUSA300_RS07800	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (3/3);  total (3/3)

TGTTGACGCAAGTAGCAGTTTTTTTATTCTTCATAAAAGTATTCTTTGTAGTATATGAATGTTGCGATACTTGCGAATCCCGCAATTGACCATGCTGTAGTGAAGTACAGCAATGGCATAAGCACAATCGCTAAGACTGTGAAGCACAGTACTGCTATTAATTAGCTTTTATATGTGTCGCTCATTTAAT  >  NC_007793/1586487‑1586676                                                                                                |                                                                                               cattgccgtgaGTAACAGTTTTTTTATTCTTCATAAAAGTATTCCTTATAAAATATGAATGTTGCGATACTTGCGAATCCCGCAATCGACCATGCAGTAg                                                                                            >  1:3023882/11‑100 (MQ=17)  attgccgtgaGTAACAGTTTTTTTATTCTTCATAAAAGTATTCCTTATAAAATATGAATGTTGCGATACTTGCGAATCCCGCAATCGACCATGCagtagt                                                                                           >  1:5916861/10‑100 (MQ=17)   ttgccgtgaGTAACAGTTTTTTTATTCTTCATAAAAGTATTCCTTATAAAATATGAATGTTGCGATACTTGCGAATCCCGCAATCGACCATGCAGTAGTg                                                                                          <  1:4273277/92‑1 (MQ=17)                                   aaaaGTATTCCTTATAAAATATGAATGTTGCGATACTTGCGAATCCCGCAATCGACCATGCAGTAGTGAAGTATAGAAACGGCATAAGTACAATTGCTag                                                          <  1:3082542/100‑2 (MQ=255)                                                                 cGATACTTGCGAATCCCGCAATCGACCATGCAGTAGTGAAGTATAGAAACGGCATAAGTACAATTGCTAAGACTGTAAAGCACAGTACTGctactaggta                            <  1:4491382/100‑5 (MQ=255)                                                                                         aCCATGCAGTAGTGAAGTATAGAAACGGCATAAGTACAATTGCTAAGACTGTAAAGCACAGTACTGCTACTAGGTAGCTTTTATAAATGTTGCTCAtttt    >  1:1725451/1‑99 (MQ=255)                                                                                           cctGCAGTAGTGAAGTATAGAAACGGCATAAGTACAATTGCTAAGACTGTAAAGCACAGTACTGCTACTAGGTAGCTTTTATAAATGTTGCTCATTTTat  >  1:87325/3‑100 (MQ=255)                                                                                                |                                                                                               TGTTGACGCAAGTAGCAGTTTTTTTATTCTTCATAAAAGTATTCTTTGTAGTATATGAATGTTGCGATACTTGCGAATCCCGCAATTGACCATGCTGTAGTGAAGTACAGCAATGGCATAAGCACAATCGCTAAGACTGTGAAGCACAGTACTGCTATTAATTAGCTTTTATATGTGTCGCTCATTTAAT  >  NC_007793/1586487‑1586676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑