Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	267,106	G→A	R235H (CGT→CAT)	SAUSA300_RS01180 →	coagulase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	267,106	0	G	A	100.0%	172.6 / NA	63	R235H (CGT→CAT)	SAUSA300_RS01180	coagulase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (57/6);  total (57/6)

TTATGGGGAGCACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACGTATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATA  >  NC_007793/267020‑267201                                                                                       |                                                                                                ttATGGGGAGCACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAgaagaa                                                                                    <  1:6582180/100‑1 (MQ=255) ttATGGGGAGCACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGaa                                                                                    <  1:5380841/100‑1 (MQ=255) ctATGGGGAGCACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGaa                                                                                    >  1:3879109/2‑100 (MQ=255)  tATGGGGAGCACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGaaa                                                                                   >  1:6306365/1‑100 (MQ=255)  caTGGGGAGCACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGaaa                                                                                   >  1:3967628/2‑100 (MQ=255)   gcggggAGCACGCGAAAGAGTTACGTGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAGATTACAAATGAACATATTAAAAAAGAAAt                                                                                  >  1:4400434/3‑100 (MQ=255)   aTGGGGAGCACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAAt                                                                                  >  1:260830/1‑100 (MQ=255)         aGCACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAGAAGaaagatcgga                                                                            >  1:2109755/1‑93 (MQ=255)          gaccgcgAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAAAAAagatcgga                                                                           >  1:1273749/4‑96 (MQ=255)          ccACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAAtgattgat                                                                           >  1:3169489/2‑100 (MQ=255)           cccgcgAAAGTGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATtgatg                                                                          >  1:1893819/3‑100 (MQ=255)           cACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATtgatg                                                                          >  1:1413928/1‑100 (MQ=255)            ccgcgAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATtgatga                                                                         >  1:5263585/2‑100 (MQ=255)                                aaaCTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATtgatg                                                     >  1:2295900/1‑100 (MQ=255)                                           aaTCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAGCATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATg                                          >  1:815684/1‑100 (MQ=255)                                           aaTCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATg                                          >  1:4728935/1‑100 (MQ=255)                                           aaTCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATg                                          >  1:4551200/1‑100 (MQ=255)                                           aaTCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATg                                          >  1:73825/1‑100 (MQ=255)                                            catcctGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATCGATGACTTAAATTCAATTATTGATGATTTCTTTATgg                                         >  1:5570539/6‑100 (MQ=255)                                             tCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGa                                        >  1:3167238/1‑100 (MQ=255)                                                 tGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACt                                    >  1:4106031/1‑100 (MQ=255)                                                 tGGAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACt                                    >  1:1490714/1‑100 (MQ=255)                                                  ggAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTa                                   >  1:3541073/1‑100 (MQ=255)                                                  ggAGATACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTCCTTTATGGAAACTa                                   >  1:3718851/1‑100 (MQ=255)                                                   tagaTACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTaa                                  >  1:852086/2‑100 (MQ=255)                                                     gtgaCAGACAATCCACATAAAATTACAAATGAACATATTAAAACAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTacac                                >  1:273404/4‑100 (MQ=255)                                                     gaTACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAAc                                >  1:645131/1‑100 (MQ=255)                                                     gaTACAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAAc                                >  1:4859815/1‑100 (MQ=255)                                                       tACAGACAATCCACATAAAATTACAGATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTGTGGAAACTAAACaa                              >  1:4842555/1‑100 (MQ=255)                                                       gaCAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACaa                              >  1:4406816/2‑100 (MQ=255)                                                        aCAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACata                             >  1:202015/1‑98 (MQ=255)                                                        aCAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACaaa                             >  1:1994668/1‑100 (MQ=255)                                                         cAGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACaaaa                            >  1:5149958/1‑100 (MQ=255)                                                          tcacccgccACATAAAATTACAAATGAACATATTAAAAAGGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAAt                           <  1:2585837/93‑1 (MQ=255)                                                          aGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAAt                           <  1:3667116/100‑1 (MQ=255)                                                          aGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAAt                           <  1:5356469/100‑1 (MQ=255)                                                          aGACAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAAt                           <  1:1927635/100‑1 (MQ=255)                                                            gcAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAg                         >  1:6163189/2‑100 (MQ=255)                                                             cAATCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAACCTAAACAAAATAGa                        >  1:4936704/1‑100 (MQ=255)                                                              caTCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGAc                       >  1:4727902/2‑100 (MQ=255)                                                              aaTCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGAc                       >  1:4070152/1‑100 (MQ=255)                                                              aaTCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAAAAAAATAGat                       >  1:5428163/1‑99 (MQ=255)                                                               aTCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGatc                      >  1:1354006/1‑98 (MQ=255)                                                               aTCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGAcc                      >  1:867352/1‑100 (MQ=255)                                                               aTCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGAcc                      >  1:3919023/1‑100 (MQ=255)                                                               aTCCACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATCGATGATTTCTTTATGGAAACTAAACAAAATAGAcc                      >  1:4304810/1‑100 (MQ=255)                                                                accACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCg                     >  1:313995/2‑100 (MQ=255)                                                                 ccACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGATCgg                    >  1:5049829/1‑99 (MQ=255)                                                                 ccACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGa                    >  1:6055198/1‑100 (MQ=255)                                                                 ccACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGa                    >  1:3209229/1‑100 (MQ=255)                                                                 ccACATAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAACAGATCgg                    >  1:270147/1‑99 (MQ=255)                                                                   acaTAAAATTACAAATGAACATATTAAACAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGaaa                  >  1:1420147/1‑100 (MQ=255)                                                                   acaTAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGATCGgaa                  >  1:3098591/1‑100 (MQ=255)                                                                   acaTAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGaaa                  >  1:374430/1‑100 (MQ=255)                                                                   acaTAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGaaa                  >  1:734224/1‑100 (MQ=255)                                                                   acaTAAAATTACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGaaa                  >  1:1821335/1‑100 (MQ=255)                                                                           ctACAAATGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAAc          >  1:3618182/2‑100 (MQ=255)                                                                                  tGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAatat   >  1:3800167/1‑100 (MQ=255)                                                                                  tGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAatat   >  1:2984355/1‑100 (MQ=255)                                                                                  tGAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAatat   >  1:1594557/1‑100 (MQ=255)                                                                                   gAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTTTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAAtata  >  1:19701/1‑100 (MQ=255)                                                                                   gAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAATGAAAtata  >  1:2673227/1‑100 (MQ=255)                                                                                   gAACATATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAataga  >  1:2530841/1‑98 (MQ=255)                                                                                       |                                                                                                TTATGGGGAGCACGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACAGACAATCCACATAAAATTACAAATGAACGTATTAAAAAAGAAATGATTGATGACTTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATA  >  NC_007793/267020‑267201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑