Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,580,734	C→T	A22A (GCG→GCA)	SAUSA300_RS07750 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,580,734	0	C	T	85.0%	34.0 / ‑0.8	20	A22A (GCG→GCA)	SAUSA300_RS07750	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base T (1/16);  total (1/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01

TTGCATTACCTATTGCGAATGCTAATTGTGGTGGATACTGTGCCGTAACTTGTTCGATGAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCAGGTTGTTTAACATTGTCTTGTGTTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACATTTGG  >  NC_007793/1580640‑1580829                                                                                               |                                                                                                ttGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTgg                                                                                            <  1:2799314/100‑1 (MQ=255) ttGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTgg                                                                                            <  1:3671227/100‑1 (MQ=255)  tGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGgt                                                                                           <  1:4267203/100‑1 (MQ=255)                             tggtggGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATttcattc                                                                <  1:2660787/100‑1 (MQ=255)                                        gtgCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACttt                                                     <  1:3126374/100‑1 (MQ=255)                                           ccGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCt                                                  <  1:2078570/100‑1 (MQ=255)                                              tAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGtt                                               <  1:2834991/100‑1 (MQ=255)                                                aCTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTAc                                             <  1:6065232/100‑1 (MQ=255)                                                  ttGTTCTATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCCGGTTGCTTAACATTGTCTTGCGTTTTGTTCATATCTACTTTTCTGTTACTg                                           <  1:2541841/100‑1 (MQ=255)                                                  ttGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTg                                           <  1:6548683/100‑1 (MQ=255)                                                  ttGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTg                                           <  1:5751879/100‑1 (MQ=255)                                                     ttCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGAtt                                        <  1:1804458/100‑1 (MQ=255)                                                     ttCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCCGGTTGCTTAACATTGTCTTGCGTTTTGTTCATATCTACTTTTCTGTTACTGAtt                                        <  1:709153/100‑1 (MQ=255)                                                              aTCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCAtt                               <  1:1173737/100‑1 (MQ=255)                                                                         cAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACta                    <  1:5932074/100‑2 (MQ=255)                                                                           aTGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCa                  <  1:5584484/100‑1 (MQ=255)                                                                                cccGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCt             >  1:1832806/2‑100 (MQ=255)                                                                                        tgtAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACAtt     <  1:4114413/100‑1 (MQ=255)                                                                                          tAATGCGCCGGTTGCTTAACATTGTCTTGCGTTTTGTTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACATTTg   <  1:5425144/100‑1 (MQ=255)                                                                                           aaTGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACagcgag  <  1:2799928/100‑6 (MQ=255)                                                                                               |                                                                                                TTGCATTACCTATTGCGAATGCTAATTGTGGTGGATACTGTGCCGTAACTTGTTCGATGAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCAGGTTGTTTAACATTGTCTTGTGTTTCATTCATATCTACTTTTCTGTTACTGATTATGCTCATTATGCTTCACTCCATTTCTTGAACATTTGG  >  NC_007793/1580640‑1580829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑