Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,580,698	G→A	F34F (TTC→TTT)	SAUSA300_RS07750 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,580,698	0	G	A	100.0%	59.5 / NA	23	F34F (TTC→TTT)	SAUSA300_RS07750	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/23);  total (0/23)

TAACGGTGCTCTAGACAAGTATTTGATTGCATTACCTATTGCGAATGCTAATTGTGGTGGATACTGTGCCGTAACTTGTTCGATGAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCAGGTTGTTTAACATTGTCTTGTGTTTCATTCATATCTACTTTTCTGTTACTGATT  >  NC_007793/1580614‑1580791                                                                                     |                                                                                              tAACGGTGCTCTAGACAAGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCa                                                                                <  1:3991288/100‑1 (MQ=255)              gACAAGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTAtg                                                                   <  1:25278/100‑1 (MQ=255)               ctaggTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAAACTATAATTTCAATGTCGCCGTAtgt                                                                  <  1:4392507/96‑1 (MQ=255)                cAAGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTAtgcg                                                                 <  1:1512488/100‑3 (MQ=255)                 aaGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATgtgt                                                                <  1:1544237/100‑1 (MQ=255)                 aaGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATgtgt                                                                <  1:2870058/100‑1 (MQ=255)                  aGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTa                                                               <  1:4371998/100‑1 (MQ=255)                  aGTAATTAATTGCATTGCCTATTGCGAATGCTAATTGTGGTGGGTCCTGTGCCGTAAATTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTa                                                               <  1:195713/100‑1 (MQ=255)                   gTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAGATCTATAATTTCAATGTCGCCGTATGTGTat                                                              <  1:1519736/100‑2 (MQ=255)                      tttAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATga                                                           <  1:32965/100‑2 (MQ=255)                           ttGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTgg                                                      <  1:2799314/100‑1 (MQ=255)                           ttGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTgg                                                      <  1:3671227/100‑1 (MQ=255)                            tGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGgt                                                     <  1:4267203/100‑1 (MQ=255)                                                       tggtggGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATttcattc                          <  1:2660787/100‑1 (MQ=255)                                                                  gtgCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACttt               <  1:3126374/100‑1 (MQ=255)                                                                     ccGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCt            <  1:2078570/100‑1 (MQ=255)                                                                        tAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGtt         <  1:2834991/100‑1 (MQ=255)                                                                          aCTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTAc       <  1:6065232/100‑1 (MQ=255)                                                                            ttGTTCTATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCCGGTTGCTTAACATTGTCTTGCGTTTTGTTCATATCTACTTTTCTGTTACTg     <  1:2541841/100‑1 (MQ=255)                                                                            ttGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTg     <  1:5751879/100‑1 (MQ=255)                                                                            ttGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTg     <  1:6548683/100‑1 (MQ=255)                                                                               ttCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGTGCTGGTTGCTTAACATTGTCTTGCATTTCATTCATATCTACTTTTCTGTTACTGAtt  <  1:1804458/100‑1 (MQ=255)                                                                               ttCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCCGGTTGCTTAACATTGTCTTGCGTTTTGTTCATATCTACTTTTCTGTTACTGAtt  <  1:709153/100‑1 (MQ=255)                                                                                     |                                                                                              TAACGGTGCTCTAGACAAGTATTTGATTGCATTACCTATTGCGAATGCTAATTGTGGTGGATACTGTGCCGTAACTTGTTCGATGAAATCTATAATTTCAATGTCGCCGTATGTGTAATGCGCAGGTTGTTTAACATTGTCTTGTGTTTCATTCATATCTACTTTTCTGTTACTGATT  >  NC_007793/1580614‑1580791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑