Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,580,638	G→A	I54I (ATC→ATT)	SAUSA300_RS07750 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,580,638	0	G	A	100.0%	40.4 / NA	16	I54I (ATC→ATT)	SAUSA300_RS07750	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/14);  total (2/14)

CCCTCCCACAAGTCAAACACTCTATCGACGTAAAACTTCGCCTTTGCTAAATCCTCATGACCATTCTTTAACGGTGCTCTAGACAAGTATTTGATTGCATTACCTATTGCGAATGCTAATTGTGGTGGATACTGTGCCGTAACTTGTTCGATGAAATCTATAATTTCAATGTCGCCGTATGTGTAATGC  >  NC_007793/1580546‑1580734                                                                                             |                                                                                                 atcTCCCGCGAATCAAATGCTCTATCGACATAAAACTTCGCTTTCGCCATATCCTCATGACCATTCTTTAACGGTGCTCTAGACAAGTATTTAATTGCAt                                                                                           <  1:1238899/98‑1 (MQ=255)  acTCCCACAAATCAAATGCTCTATCGACATAAAACTTCGCTTTCGCCATATCCTCATGACCATTCTTTAACGGTGCTCTAGACAAGTATTTAATTGCAtt                                                                                          >  1:6577749/2‑100 (MQ=255)          aaatCAAATGCTCTATCGACATAAAACTTCGCTTTCGCCATATCCTCATGACCATTCTTTAACGGTGCTCTAGACAAGTATTTAATTGCATTACCTATTg                                                                                  <  1:5066514/97‑1 (MQ=255)            atCAAATGCTCTATCGACATAAAACTTCGCTTTCGCCATATCCTCATGACCATTCTTTAACGGTGCTCTAGACAAGTATTTAATTGCATTACCTATTGCg                                                                                <  1:36041/99‑1 (MQ=255)                                             cgccataTCCTCATGACCATTCTTTAACGGTGCTCTAGACAAGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACt                                               <  1:2577533/94‑1 (MQ=255)                                                       tCATGACCATTCTTTAACGGTGCTCTAGACAAGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAAtaa                                     >  1:6005339/1‑98 (MQ=255)                                                                     tAACGGTGCTCTAGACAAGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCa                       <  1:3991288/100‑1 (MQ=255)                                                                                  gACAAGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTAtg          <  1:25278/100‑1 (MQ=255)                                                                                   ctaggTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAAACTATAATTTCAATGTCGCCGTAtgt         <  1:4392507/96‑1 (MQ=255)                                                                                    cAAGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTAtgcg        <  1:1512488/100‑3 (MQ=255)                                                                                     aaGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATgtgt       <  1:1544237/100‑1 (MQ=255)                                                                                     aaGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATgtgt       <  1:2870058/100‑1 (MQ=255)                                                                                      aGTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTa      <  1:4371998/100‑1 (MQ=255)                                                                                      aGTAATTAATTGCATTGCCTATTGCGAATGCTAATTGTGGTGGGTCCTGTGCCGTAAATTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTa      <  1:195713/100‑1 (MQ=255)                                                                                       gTATTTAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAGATCTATAATTTCAATGTCGCCGTATGTGTat     <  1:1519736/100‑2 (MQ=255)                                                                                          tttAATTGCATTACCTATTGCGAATGCTAATTGTGGTGGGTACTGTGCCGTAACTTGTTCAATAAAATCTATAATTTCAATGTCGCCGTATGTGTAATga  <  1:32965/100‑2 (MQ=255)                                                                                             |                                                                                                 CCCTCCCACAAGTCAAACACTCTATCGACGTAAAACTTCGCCTTTGCTAAATCCTCATGACCATTCTTTAACGGTGCTCTAGACAAGTATTTGATTGCATTACCTATTGCGAATGCTAATTGTGGTGGATACTGTGCCGTAACTTGTTCGATGAAATCTATAATTTCAATGTCGCCGTATGTGTAATGC  >  NC_007793/1580546‑1580734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑