Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,580,274	T→C	K17K (AAA→AAG)	SAUSA300_RS07740 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,580,274	0	T	C	100.0%	10.2 / NA	9	K17K (AAA→AAG)	SAUSA300_RS07740	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/9);  total (0/9)

GTAATACGTGCCATTTACTACATGAGTATGTGCCACTCGTTCGTTATCCTGATACAGATATCTTTTAGAGCCGAAAAATTGGTTTAAGCGTTCTTTACGTGCGCTATCTGTCATGGTCGTCACTCCTTTTAACAATTAGGCAGTCCAAA  >  NC_007793/1580211‑1580359                                                                |                                                                                      gTAATAAGTGTCATTTAATACATGGATATGTGCCACTCGCTCGTTATTCTGATACAGATATCTCTTAGAACCGAAAAATTGGTTTAAGTATTCTTTACgg                                                   <  1:6414665/100‑2 (MQ=11)      aaatgtcATTCACTACATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCTCTTAGATCCGAAAAATTGATTTAAGTATTCTTtgcgtgcgtt                                              <  1:3572511/94‑3 (MQ=14)      aaatgtcATTCACTACATGGATATGTGCCACTCGTTCGTTATCCCGATACAGATATCTCTTAGATCCGAAAAATTGATTTAAGTATTCTTtgcgtgcgtt                                              <  1:6077066/94‑3 (MQ=14)                 aatacATGGATATGTGCCACTCGCTCGTTATTCTGATACAGATATCTCTTAGAACCGAAAAATTGGTTTAAGTATTCTTTACGTGCGCTATCTGTCATgt                                   <  1:628996/98‑2 (MQ=11)                 aatacATGGATATGTGCCACTCGCTCGTTATTCTGATACAGATATCTCTTAGAACCGAAAAATTGGTTTAAGTATTCTTTACGTGCGCTATCTGTCATgc                                   <  1:2715311/98‑2 (MQ=11)                    acATGGATATGTGCCACTCGCTCGTTATTCTGATACAGATATCTCTTAGAACCGAAAAATTGGTTTAAGTATTCTTTACGTGCGCTATCTGTCATgtcca                                <  1:5762924/100‑5 (MQ=11)                                          cgttATCCCGATACAGATATCTCTTAGATCCGAAAAATTGATTTAAGTATTCTTTGCGTGCGTTATCTGTCATAGTCATCACTCCTTTTAACAATTAggt          <  1:3963284/100‑2 (MQ=14)                                          cgttATCCCGATACAGATATCTCTTAGATCCGAAAAATTGATTTAAGTATTCTTTGCGTGCGTTATCTGTCATAGTCATCACTCCTTTTAACAATTAggt          <  1:791404/100‑2 (MQ=14)                                                  cgATACAGATATCTCTTAGATCCGAAAAATTGATTTAAGTATTCTTTGCGTGCGTTATCTGTCATAGTCATCACTCCTTTTAACAATTAGGCAGACCaag  <  1:6578349/99‑2 (MQ=14)                                                                |                                                                                      GTAATACGTGCCATTTACTACATGAGTATGTGCCACTCGTTCGTTATCCTGATACAGATATCTTTTAGAGCCGAAAAATTGGTTTAAGCGTTCTTTACGTGCGCTATCTGTCATGGTCGTCACTCCTTTTAACAATTAGGCAGTCCAAA  >  NC_007793/1580211‑1580359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑