Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,579,641	A→G	D62D (GAT→GAC)	SAUSA300_RS07730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,579,641	0	A	G	100.0%	12.0 / NA	7	D62D (GAT→GAC)	SAUSA300_RS07730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/7);  total (0/7)

CTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCCGCTAATTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATT  >  NC_007793/1579553‑1579694                                                                                         |                                                      cttcttTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCAGCTAATTCATCAAGTTGTACGTCTAACGGCtt                                            <  1:202176/100‑1 (MQ=255)  tcttccgaTCTC‑TCTGATGACACTCCTACTTGATTCGCAATACCCAATCCAAACGCCAACATGTCAGCTAATTCATCAAGTTGTACGTCTAACGGCTTag                                          <  1:2021620/93‑2 (MQ=255)  cagacggtgctctTCCGATCTCGCTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCAGCTAATTCATCAAGTTGTACGTCTAACGGCttt                                           <  1:4128208/91‑2 (MQ=255)            tcttctGATGACACTCCTACTTGATTCGCAATACTCAATCCAGACGCCAACATGTCAGCTAATTCATCAAGTTGTACGTCTGACGGCTTACCTGGTttct                                 <  1:3644552/100‑1 (MQ=255)                        tcTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCAGCTAATTCATCAAGTTGTACGTCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCt                     <  1:5507735/99‑1 (MQ=255)                                   aTTAGCAATACTCAATCCAAACGCTAACATGTCTGCTAACTCGTCTAGCTGTACGTCTAACGGTTTACCTGGTTTCTTCTTCCAATTCTTGAACGTTTcc          <  1:4761239/100‑1 (MQ=255)                                           tACTCAATCCAAGCGCCAACATGTCAGCTAATTCATCAAGTTGTACGTCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTAtt  <  1:1608096/100‑1 (MQ=255)                                                                                         |                                                      CTTCTTTTATCTCTTCTGATGACACTCCTACTTGATTCGCAATACTCAATCCAAACGCCAACATGTCCGCTAATTCATCTAACTGAACATCTAACGGCTTACCTGGTTTCTTCTTCCAGTTCTTAAACGTTTCCAATGTATT  >  NC_007793/1579553‑1579694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑